@article{b53c88dd0d524dd1973f7e070671405a,
title = "Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins",
abstract = "We describe the phenotype of mice lacking both endothelial selectins after sequential ablation of the genes encoding P- and E-selectins. In contrast with the rather mild phenotypes observed in mice deficient in a single selectin gene, the doubly deficient mice present extreme leukocytosis, elevated cytokine levels, and alterations in hematopoiesis. Granulocytopoiesis is increased both in bone marrow and spleen, while erythropoiesis is partially translocated to the spleen. Virtual lack of leukocyte rolling and low extravasation at sites of inflammation make these animals susceptible to opportunistic bacterial infections, to which they succumb. Our results show that the absence of endothelial selectins severely affects leukocyte homeostasis and indicate that these two selectins are as important for normal leukocyte function as are the leukocyte β2 integrins.",
author = "Frenette, {Paul S.} and Mayadas, {Tanya N.} and Helen Rayburn and Hynes, {Richard O.} and Wagner, {Denisa D.}",
note = "Funding Information: Correspondence should be addressed to D. D. W. We are grateful to Mollie Ullmann-Culler{\'e} for mouse husbandry, Kim Mercer, Marge Cummiskey, Jane E. Trevithick, Caitlin Moyna, and Simin Saffaripour for technical assistance, and Glen Paradis for flow cytometry analysis. We thank Drs. Robert S. Schwartz, John P. Manis, and Jose Carlos Gutierrez-Ramos for helpful discussions, Joan Lane and Roderick Bronson for histologic interpretations, and Ian J. Webb for advice in CFU assays. Special thanks go to Dr. Robert S. Schwartz for providing critical comments on this manuscript. This work was supported by National Institutes of Health grants HL41002 and HL53756 (D. D. W.) and PO1 HL41484 (R. O. H.). R. O. H. is an Investigator of the Howard Hughes Medical Institute. P. S. F. is a postdoctoral fellow supported in part by a Ciba-Geigy Gabbour-Labb{\'e} fellowship awarded by the National Cancer Institute of Canada and, later, by a fellowship from the Medical Research Council of Canada.",
year = "1996",
month = feb,
day = "23",
doi = "10.1016/S0092-8674(00)81032-6",
language = "English (US)",
volume = "84",
pages = "563--574",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",
}