Abstract
We describe the phenotype of mice lacking both endothelial selectins after sequential ablation of the genes encoding P- and E-selectins. In contrast with the rather mild phenotypes observed in mice deficient in a single selectin gene, the doubly deficient mice present extreme leukocytosis, elevated cytokine levels, and alterations in hematopoiesis. Granulocytopoiesis is increased both in bone marrow and spleen, while erythropoiesis is partially translocated to the spleen. Virtual lack of leukocyte rolling and low extravasation at sites of inflammation make these animals susceptible to opportunistic bacterial infections, to which they succumb. Our results show that the absence of endothelial selectins severely affects leukocyte homeostasis and indicate that these two selectins are as important for normal leukocyte function as are the leukocyte β2 integrins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 563-574 |
| Number of pages | 12 |
| Journal | Cell |
| Volume | 84 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 23 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. / Frenette, Paul S.; Mayadas, Tanya N.; Rayburn, Helen; Hynes, Richard O.; Wagner, Denisa D.
In: Cell, Vol. 84, No. 4, 23.02.1996, p. 563-574.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins
AU - Frenette, Paul S.
AU - Mayadas, Tanya N.
AU - Rayburn, Helen
AU - Hynes, Richard O.
AU - Wagner, Denisa D.
PY - 1996/2/23
Y1 - 1996/2/23
N2 - We describe the phenotype of mice lacking both endothelial selectins after sequential ablation of the genes encoding P- and E-selectins. In contrast with the rather mild phenotypes observed in mice deficient in a single selectin gene, the doubly deficient mice present extreme leukocytosis, elevated cytokine levels, and alterations in hematopoiesis. Granulocytopoiesis is increased both in bone marrow and spleen, while erythropoiesis is partially translocated to the spleen. Virtual lack of leukocyte rolling and low extravasation at sites of inflammation make these animals susceptible to opportunistic bacterial infections, to which they succumb. Our results show that the absence of endothelial selectins severely affects leukocyte homeostasis and indicate that these two selectins are as important for normal leukocyte function as are the leukocyte β2 integrins.
AB - We describe the phenotype of mice lacking both endothelial selectins after sequential ablation of the genes encoding P- and E-selectins. In contrast with the rather mild phenotypes observed in mice deficient in a single selectin gene, the doubly deficient mice present extreme leukocytosis, elevated cytokine levels, and alterations in hematopoiesis. Granulocytopoiesis is increased both in bone marrow and spleen, while erythropoiesis is partially translocated to the spleen. Virtual lack of leukocyte rolling and low extravasation at sites of inflammation make these animals susceptible to opportunistic bacterial infections, to which they succumb. Our results show that the absence of endothelial selectins severely affects leukocyte homeostasis and indicate that these two selectins are as important for normal leukocyte function as are the leukocyte β2 integrins.
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U2 - 10.1016/S0092-8674(00)81032-6
DO - 10.1016/S0092-8674(00)81032-6
M3 - Article
VL - 84
SP - 563
EP - 574
JO - Cell
JF - Cell
SN - 0092-8674
IS - 4
ER -