Susceptibility of gram-positive and -negative bacteria to novel nitric oxide-releasing nanoparticle technology

Adam Friedman, Karin Blecher Paz, David Sanchez, Chaim Tuckman-Vernon, Philip Gialanella, Joel M. Friedman, Luis R. Martinez, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The rapidly evolving crisis of antibiotic resistance among microorganisms has contributed to the rise of patient morbidity and mortality from nosocomial and community-acquired infections. Therefore, innovative antimicrobial technology targeting mechanisms to which the bacteria are unlikely to evolve resistance is urgently needed. We have previously described a nitric oxide-releasing nanoparticle (NO-np) with efficacy against methicillin-resistant Staphylococcus aureus (MRsa) and Acinetobacter baumannii in vitro and in murine wound and abscess models. although the prior findings suggest that the NO-np can be a useful therapeutic for skin and soft tissue infections, the antimicrobial spectrum of NO-np has yet to be fully elucidated. In the current study, we investigated the efficacy of a NO-np against several Gram-positive and -negative clinical isolates. We found that the NO-np were uniformly active against all of the Streptococcus pyogenes, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa clinical isolates examined, including strains that were both sensitive and resistant to commonly used antibiotics. We concluded that the NO-np have the potential to serve as a novel broad spectrum antimicrobial agent.

Original languageEnglish (US)
JournalVirulence
Volume2
Issue number3
StatePublished - May 2011

Fingerprint

Gram-Positive Bacteria
Gram-Negative Bacteria
Nanoparticles
Nitric Oxide
Technology
Community-Acquired Infections
Acinetobacter baumannii
Soft Tissue Infections
Enterococcus faecalis
Streptococcus pyogenes
Klebsiella pneumoniae
Methicillin-Resistant Staphylococcus aureus
Microbial Drug Resistance
Anti-Infective Agents
Abscess
Pseudomonas aeruginosa
Escherichia coli
Anti-Bacterial Agents
Morbidity
Bacteria

Keywords

  • Antibacterial
  • Gram-negative
  • Gram-positive
  • Nanotechnology
  • Nitric oxide

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology
  • Parasitology
  • Immunology
  • Microbiology (medical)

Cite this

Susceptibility of gram-positive and -negative bacteria to novel nitric oxide-releasing nanoparticle technology. / Friedman, Adam; Blecher Paz, Karin; Sanchez, David; Tuckman-Vernon, Chaim; Gialanella, Philip; Friedman, Joel M.; Martinez, Luis R.; Nosanchuk, Joshua D.

In: Virulence, Vol. 2, No. 3, 05.2011.

Research output: Contribution to journalArticle

@article{1285dbd1eacd426e84e6e7280aa4bd31,
title = "Susceptibility of gram-positive and -negative bacteria to novel nitric oxide-releasing nanoparticle technology",
abstract = "The rapidly evolving crisis of antibiotic resistance among microorganisms has contributed to the rise of patient morbidity and mortality from nosocomial and community-acquired infections. Therefore, innovative antimicrobial technology targeting mechanisms to which the bacteria are unlikely to evolve resistance is urgently needed. We have previously described a nitric oxide-releasing nanoparticle (NO-np) with efficacy against methicillin-resistant Staphylococcus aureus (MRsa) and Acinetobacter baumannii in vitro and in murine wound and abscess models. although the prior findings suggest that the NO-np can be a useful therapeutic for skin and soft tissue infections, the antimicrobial spectrum of NO-np has yet to be fully elucidated. In the current study, we investigated the efficacy of a NO-np against several Gram-positive and -negative clinical isolates. We found that the NO-np were uniformly active against all of the Streptococcus pyogenes, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa clinical isolates examined, including strains that were both sensitive and resistant to commonly used antibiotics. We concluded that the NO-np have the potential to serve as a novel broad spectrum antimicrobial agent.",
keywords = "Antibacterial, Gram-negative, Gram-positive, Nanotechnology, Nitric oxide",
author = "Adam Friedman and {Blecher Paz}, Karin and David Sanchez and Chaim Tuckman-Vernon and Philip Gialanella and Friedman, {Joel M.} and Martinez, {Luis R.} and Nosanchuk, {Joshua D.}",
year = "2011",
month = "5",
language = "English (US)",
volume = "2",
journal = "Virulence",
issn = "2150-5594",
publisher = "Landes Bioscience",
number = "3",

}

TY - JOUR

T1 - Susceptibility of gram-positive and -negative bacteria to novel nitric oxide-releasing nanoparticle technology

AU - Friedman, Adam

AU - Blecher Paz, Karin

AU - Sanchez, David

AU - Tuckman-Vernon, Chaim

AU - Gialanella, Philip

AU - Friedman, Joel M.

AU - Martinez, Luis R.

AU - Nosanchuk, Joshua D.

PY - 2011/5

Y1 - 2011/5

N2 - The rapidly evolving crisis of antibiotic resistance among microorganisms has contributed to the rise of patient morbidity and mortality from nosocomial and community-acquired infections. Therefore, innovative antimicrobial technology targeting mechanisms to which the bacteria are unlikely to evolve resistance is urgently needed. We have previously described a nitric oxide-releasing nanoparticle (NO-np) with efficacy against methicillin-resistant Staphylococcus aureus (MRsa) and Acinetobacter baumannii in vitro and in murine wound and abscess models. although the prior findings suggest that the NO-np can be a useful therapeutic for skin and soft tissue infections, the antimicrobial spectrum of NO-np has yet to be fully elucidated. In the current study, we investigated the efficacy of a NO-np against several Gram-positive and -negative clinical isolates. We found that the NO-np were uniformly active against all of the Streptococcus pyogenes, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa clinical isolates examined, including strains that were both sensitive and resistant to commonly used antibiotics. We concluded that the NO-np have the potential to serve as a novel broad spectrum antimicrobial agent.

AB - The rapidly evolving crisis of antibiotic resistance among microorganisms has contributed to the rise of patient morbidity and mortality from nosocomial and community-acquired infections. Therefore, innovative antimicrobial technology targeting mechanisms to which the bacteria are unlikely to evolve resistance is urgently needed. We have previously described a nitric oxide-releasing nanoparticle (NO-np) with efficacy against methicillin-resistant Staphylococcus aureus (MRsa) and Acinetobacter baumannii in vitro and in murine wound and abscess models. although the prior findings suggest that the NO-np can be a useful therapeutic for skin and soft tissue infections, the antimicrobial spectrum of NO-np has yet to be fully elucidated. In the current study, we investigated the efficacy of a NO-np against several Gram-positive and -negative clinical isolates. We found that the NO-np were uniformly active against all of the Streptococcus pyogenes, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa clinical isolates examined, including strains that were both sensitive and resistant to commonly used antibiotics. We concluded that the NO-np have the potential to serve as a novel broad spectrum antimicrobial agent.

KW - Antibacterial

KW - Gram-negative

KW - Gram-positive

KW - Nanotechnology

KW - Nitric oxide

UR - http://www.scopus.com/inward/record.url?scp=79959231672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959231672&partnerID=8YFLogxK

M3 - Article

VL - 2

JO - Virulence

JF - Virulence

SN - 2150-5594

IS - 3

ER -