Survival of mice infected with Mycobacterium smegmatis containing large DNA fragments from Mycobacterium tuberculosis

F. C. Bange, F. M. Collins, W. R. Jacobs

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Mycobacterium smegmatis is typically used as a bacterial host for cloning and expressing single genes or genomic libraries of the human pathogen Mycobacterium tuberculosis. To study virulence of M. tuberculosis, we set out to ask the question, whether a genomic library derived from M. tuberculosis H37Rv confers virulence to the non-virulent M. smegmatis. A representative library from the M. tuberculosis H37Rv genome was generated and transformed into wild-type M. smegmatis. Mice were challenged with recombinant clones by intravenous, aerogenic and intranasal infection. We were unable to detect either growth or persistence of recombinant clones in tissues of infected mice; instead, the infection was cleared. Since the concern that virulent traits might be transferred, biosafety regulations often require the handling of these experiments at Biosafety Level 3. However, we failed to find any evidence that the M. tuberculosis library confers virulence when expressed in M. smegmatis. We suggest that the results, presented here, should fundamentally alter the containment requirements for similar experiments in the future.

Original languageEnglish (US)
Pages (from-to)171-180
Number of pages10
JournalTubercle and Lung Disease
Volume79
Issue number3
DOIs
StatePublished - Jun 1999
Externally publishedYes

Fingerprint

Mycobacterium smegmatis
Mycobacterium tuberculosis
DNA
Virulence
Genomic Library
Libraries
Clone Cells
Infection
Gene Library
Organism Cloning
Genome
Growth

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Survival of mice infected with Mycobacterium smegmatis containing large DNA fragments from Mycobacterium tuberculosis. / Bange, F. C.; Collins, F. M.; Jacobs, W. R.

In: Tubercle and Lung Disease, Vol. 79, No. 3, 06.1999, p. 171-180.

Research output: Contribution to journalArticle

@article{585a7e8c747540648062e171b1a36427,
title = "Survival of mice infected with Mycobacterium smegmatis containing large DNA fragments from Mycobacterium tuberculosis",
abstract = "Mycobacterium smegmatis is typically used as a bacterial host for cloning and expressing single genes or genomic libraries of the human pathogen Mycobacterium tuberculosis. To study virulence of M. tuberculosis, we set out to ask the question, whether a genomic library derived from M. tuberculosis H37Rv confers virulence to the non-virulent M. smegmatis. A representative library from the M. tuberculosis H37Rv genome was generated and transformed into wild-type M. smegmatis. Mice were challenged with recombinant clones by intravenous, aerogenic and intranasal infection. We were unable to detect either growth or persistence of recombinant clones in tissues of infected mice; instead, the infection was cleared. Since the concern that virulent traits might be transferred, biosafety regulations often require the handling of these experiments at Biosafety Level 3. However, we failed to find any evidence that the M. tuberculosis library confers virulence when expressed in M. smegmatis. We suggest that the results, presented here, should fundamentally alter the containment requirements for similar experiments in the future.",
author = "Bange, {F. C.} and Collins, {F. M.} and Jacobs, {W. R.}",
year = "1999",
month = "6",
doi = "10.1054/tuld.1998.0201",
language = "English (US)",
volume = "79",
pages = "171--180",
journal = "Tuberculosis",
issn = "1472-9792",
publisher = "Churchill Livingstone",
number = "3",

}

TY - JOUR

T1 - Survival of mice infected with Mycobacterium smegmatis containing large DNA fragments from Mycobacterium tuberculosis

AU - Bange, F. C.

AU - Collins, F. M.

AU - Jacobs, W. R.

PY - 1999/6

Y1 - 1999/6

N2 - Mycobacterium smegmatis is typically used as a bacterial host for cloning and expressing single genes or genomic libraries of the human pathogen Mycobacterium tuberculosis. To study virulence of M. tuberculosis, we set out to ask the question, whether a genomic library derived from M. tuberculosis H37Rv confers virulence to the non-virulent M. smegmatis. A representative library from the M. tuberculosis H37Rv genome was generated and transformed into wild-type M. smegmatis. Mice were challenged with recombinant clones by intravenous, aerogenic and intranasal infection. We were unable to detect either growth or persistence of recombinant clones in tissues of infected mice; instead, the infection was cleared. Since the concern that virulent traits might be transferred, biosafety regulations often require the handling of these experiments at Biosafety Level 3. However, we failed to find any evidence that the M. tuberculosis library confers virulence when expressed in M. smegmatis. We suggest that the results, presented here, should fundamentally alter the containment requirements for similar experiments in the future.

AB - Mycobacterium smegmatis is typically used as a bacterial host for cloning and expressing single genes or genomic libraries of the human pathogen Mycobacterium tuberculosis. To study virulence of M. tuberculosis, we set out to ask the question, whether a genomic library derived from M. tuberculosis H37Rv confers virulence to the non-virulent M. smegmatis. A representative library from the M. tuberculosis H37Rv genome was generated and transformed into wild-type M. smegmatis. Mice were challenged with recombinant clones by intravenous, aerogenic and intranasal infection. We were unable to detect either growth or persistence of recombinant clones in tissues of infected mice; instead, the infection was cleared. Since the concern that virulent traits might be transferred, biosafety regulations often require the handling of these experiments at Biosafety Level 3. However, we failed to find any evidence that the M. tuberculosis library confers virulence when expressed in M. smegmatis. We suggest that the results, presented here, should fundamentally alter the containment requirements for similar experiments in the future.

UR - http://www.scopus.com/inward/record.url?scp=0345621667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345621667&partnerID=8YFLogxK

U2 - 10.1054/tuld.1998.0201

DO - 10.1054/tuld.1998.0201

M3 - Article

C2 - 10656115

AN - SCOPUS:0345621667

VL - 79

SP - 171

EP - 180

JO - Tuberculosis

JF - Tuberculosis

SN - 1472-9792

IS - 3

ER -