Survey of Florida green turtles for exposure to a disease-associated herpesvirus

Sadie S. Coberley, Lawrence H. Herbst, Llewellyn M. Ehrhart, Dean A. Bagley, Shigetomo Hirama, Elliott R. Jacobson, Paul A. Klein

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A recently developed enzyme-linked immunosorbent assay (ELISA) was used to assess exposure of Florida wild green turtles Chelonia mydas to LETV, the herpesvirus associated with lung-eye-trachea disease (LETD). Plasma samples from 329 wild juvenile green turtles netted in the Indian River lagoon, along the Sebastian reef, or in the Trident basin (Indian River and Brevard Counties, Florida) were tested by ELISA for the presence of antibodies to LETV. Plasma samples from 180 wild juvenile green turtles were tested from these study sites to compare the prevalence of anti-LETV antibodies. While some plasma samples from each site contained anti-LETV antibodies (confirmed by Western blot analysis), plasma samples collected from the Indian River lagoon had statistically higher optical density values measured in the ELISA. No statistical differences were observed when these same plasma samples were analyzed for changes in the level of anti-LETV antibodies over 3 years (1997, 1998, and 1999). To explore the relationship between anti-LETV antibodies and fibropapillomatosis (FP), plasma from 133 green turtles scored for fibropapilloma tumor severity were tested by ELISA. There was no correlation between tumor severity and the presence of antibodies against LETV. Additional plasma samples collected from 16 tagged green turtles captured and sampled more than once (recaptures) were also tested to monitor antibody levels to LETV relative to the FP status of individual turtles over time. Again there was no clear relationship between FP tumor status and the presence of antibodies to LETV. Finally, ELISA tests on plasma from 13 nesting female turtles (9 green and 4 loggerhead) revealed high levels of anti-LETV antibodies in 11 individuals, including 2 loggerhead turtles. These results provide strong evidence that wild Florida green turtle populations at these 3 study sites are exposed to LETV or a closely related virus and that loggerhead turtles may be exposed as well. Based on a cutoff optical density value of 0.310, 71 out of the 329 wild Florida green turtles tested were seropositive for LETV antibodies (seroprevalence = 21.6%). In addition, no relationship between FP tumor severity or status and the presence of anti-LETV antibodies was found, further supporting the hypothesis that LETV and the FP-associated herpesvirus (FPHV) are separate infections of marine turtles.

Original languageEnglish (US)
Pages (from-to)159-167
Number of pages9
JournalDiseases of Aquatic Organisms
Volume47
Issue number3
StatePublished - Dec 5 2001
Externally publishedYes

Fingerprint

Chelonia mydas
turtle
antibody
antibodies
plasma
enzyme-linked immunosorbent assay
Caretta caretta
tumor
assay
enzyme
turtles
neoplasms
sampling
absorbance
rivers
exposure
lagoon
eye disease
river
trachea (vertebrates)

Keywords

  • Antibodies
  • Chelonia mydas
  • Chelonian herpesviruses
  • ELISA
  • Green turtle
  • LETD
  • LETD-associated herpesvirus
  • LETV
  • Lung-eye-trachea disease

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

Coberley, S. S., Herbst, L. H., Ehrhart, L. M., Bagley, D. A., Hirama, S., Jacobson, E. R., & Klein, P. A. (2001). Survey of Florida green turtles for exposure to a disease-associated herpesvirus. Diseases of Aquatic Organisms, 47(3), 159-167.

Survey of Florida green turtles for exposure to a disease-associated herpesvirus. / Coberley, Sadie S.; Herbst, Lawrence H.; Ehrhart, Llewellyn M.; Bagley, Dean A.; Hirama, Shigetomo; Jacobson, Elliott R.; Klein, Paul A.

In: Diseases of Aquatic Organisms, Vol. 47, No. 3, 05.12.2001, p. 159-167.

Research output: Contribution to journalArticle

Coberley, SS, Herbst, LH, Ehrhart, LM, Bagley, DA, Hirama, S, Jacobson, ER & Klein, PA 2001, 'Survey of Florida green turtles for exposure to a disease-associated herpesvirus', Diseases of Aquatic Organisms, vol. 47, no. 3, pp. 159-167.
Coberley SS, Herbst LH, Ehrhart LM, Bagley DA, Hirama S, Jacobson ER et al. Survey of Florida green turtles for exposure to a disease-associated herpesvirus. Diseases of Aquatic Organisms. 2001 Dec 5;47(3):159-167.
Coberley, Sadie S. ; Herbst, Lawrence H. ; Ehrhart, Llewellyn M. ; Bagley, Dean A. ; Hirama, Shigetomo ; Jacobson, Elliott R. ; Klein, Paul A. / Survey of Florida green turtles for exposure to a disease-associated herpesvirus. In: Diseases of Aquatic Organisms. 2001 ; Vol. 47, No. 3. pp. 159-167.
@article{cf7d35f68fe3468487d384b8bcf492b4,
title = "Survey of Florida green turtles for exposure to a disease-associated herpesvirus",
abstract = "A recently developed enzyme-linked immunosorbent assay (ELISA) was used to assess exposure of Florida wild green turtles Chelonia mydas to LETV, the herpesvirus associated with lung-eye-trachea disease (LETD). Plasma samples from 329 wild juvenile green turtles netted in the Indian River lagoon, along the Sebastian reef, or in the Trident basin (Indian River and Brevard Counties, Florida) were tested by ELISA for the presence of antibodies to LETV. Plasma samples from 180 wild juvenile green turtles were tested from these study sites to compare the prevalence of anti-LETV antibodies. While some plasma samples from each site contained anti-LETV antibodies (confirmed by Western blot analysis), plasma samples collected from the Indian River lagoon had statistically higher optical density values measured in the ELISA. No statistical differences were observed when these same plasma samples were analyzed for changes in the level of anti-LETV antibodies over 3 years (1997, 1998, and 1999). To explore the relationship between anti-LETV antibodies and fibropapillomatosis (FP), plasma from 133 green turtles scored for fibropapilloma tumor severity were tested by ELISA. There was no correlation between tumor severity and the presence of antibodies against LETV. Additional plasma samples collected from 16 tagged green turtles captured and sampled more than once (recaptures) were also tested to monitor antibody levels to LETV relative to the FP status of individual turtles over time. Again there was no clear relationship between FP tumor status and the presence of antibodies to LETV. Finally, ELISA tests on plasma from 13 nesting female turtles (9 green and 4 loggerhead) revealed high levels of anti-LETV antibodies in 11 individuals, including 2 loggerhead turtles. These results provide strong evidence that wild Florida green turtle populations at these 3 study sites are exposed to LETV or a closely related virus and that loggerhead turtles may be exposed as well. Based on a cutoff optical density value of 0.310, 71 out of the 329 wild Florida green turtles tested were seropositive for LETV antibodies (seroprevalence = 21.6{\%}). In addition, no relationship between FP tumor severity or status and the presence of anti-LETV antibodies was found, further supporting the hypothesis that LETV and the FP-associated herpesvirus (FPHV) are separate infections of marine turtles.",
keywords = "Antibodies, Chelonia mydas, Chelonian herpesviruses, ELISA, Green turtle, LETD, LETD-associated herpesvirus, LETV, Lung-eye-trachea disease",
author = "Coberley, {Sadie S.} and Herbst, {Lawrence H.} and Ehrhart, {Llewellyn M.} and Bagley, {Dean A.} and Shigetomo Hirama and Jacobson, {Elliott R.} and Klein, {Paul A.}",
year = "2001",
month = "12",
day = "5",
language = "English (US)",
volume = "47",
pages = "159--167",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",
publisher = "Inter-Research",
number = "3",

}

TY - JOUR

T1 - Survey of Florida green turtles for exposure to a disease-associated herpesvirus

AU - Coberley, Sadie S.

AU - Herbst, Lawrence H.

AU - Ehrhart, Llewellyn M.

AU - Bagley, Dean A.

AU - Hirama, Shigetomo

AU - Jacobson, Elliott R.

AU - Klein, Paul A.

PY - 2001/12/5

Y1 - 2001/12/5

N2 - A recently developed enzyme-linked immunosorbent assay (ELISA) was used to assess exposure of Florida wild green turtles Chelonia mydas to LETV, the herpesvirus associated with lung-eye-trachea disease (LETD). Plasma samples from 329 wild juvenile green turtles netted in the Indian River lagoon, along the Sebastian reef, or in the Trident basin (Indian River and Brevard Counties, Florida) were tested by ELISA for the presence of antibodies to LETV. Plasma samples from 180 wild juvenile green turtles were tested from these study sites to compare the prevalence of anti-LETV antibodies. While some plasma samples from each site contained anti-LETV antibodies (confirmed by Western blot analysis), plasma samples collected from the Indian River lagoon had statistically higher optical density values measured in the ELISA. No statistical differences were observed when these same plasma samples were analyzed for changes in the level of anti-LETV antibodies over 3 years (1997, 1998, and 1999). To explore the relationship between anti-LETV antibodies and fibropapillomatosis (FP), plasma from 133 green turtles scored for fibropapilloma tumor severity were tested by ELISA. There was no correlation between tumor severity and the presence of antibodies against LETV. Additional plasma samples collected from 16 tagged green turtles captured and sampled more than once (recaptures) were also tested to monitor antibody levels to LETV relative to the FP status of individual turtles over time. Again there was no clear relationship between FP tumor status and the presence of antibodies to LETV. Finally, ELISA tests on plasma from 13 nesting female turtles (9 green and 4 loggerhead) revealed high levels of anti-LETV antibodies in 11 individuals, including 2 loggerhead turtles. These results provide strong evidence that wild Florida green turtle populations at these 3 study sites are exposed to LETV or a closely related virus and that loggerhead turtles may be exposed as well. Based on a cutoff optical density value of 0.310, 71 out of the 329 wild Florida green turtles tested were seropositive for LETV antibodies (seroprevalence = 21.6%). In addition, no relationship between FP tumor severity or status and the presence of anti-LETV antibodies was found, further supporting the hypothesis that LETV and the FP-associated herpesvirus (FPHV) are separate infections of marine turtles.

AB - A recently developed enzyme-linked immunosorbent assay (ELISA) was used to assess exposure of Florida wild green turtles Chelonia mydas to LETV, the herpesvirus associated with lung-eye-trachea disease (LETD). Plasma samples from 329 wild juvenile green turtles netted in the Indian River lagoon, along the Sebastian reef, or in the Trident basin (Indian River and Brevard Counties, Florida) were tested by ELISA for the presence of antibodies to LETV. Plasma samples from 180 wild juvenile green turtles were tested from these study sites to compare the prevalence of anti-LETV antibodies. While some plasma samples from each site contained anti-LETV antibodies (confirmed by Western blot analysis), plasma samples collected from the Indian River lagoon had statistically higher optical density values measured in the ELISA. No statistical differences were observed when these same plasma samples were analyzed for changes in the level of anti-LETV antibodies over 3 years (1997, 1998, and 1999). To explore the relationship between anti-LETV antibodies and fibropapillomatosis (FP), plasma from 133 green turtles scored for fibropapilloma tumor severity were tested by ELISA. There was no correlation between tumor severity and the presence of antibodies against LETV. Additional plasma samples collected from 16 tagged green turtles captured and sampled more than once (recaptures) were also tested to monitor antibody levels to LETV relative to the FP status of individual turtles over time. Again there was no clear relationship between FP tumor status and the presence of antibodies to LETV. Finally, ELISA tests on plasma from 13 nesting female turtles (9 green and 4 loggerhead) revealed high levels of anti-LETV antibodies in 11 individuals, including 2 loggerhead turtles. These results provide strong evidence that wild Florida green turtle populations at these 3 study sites are exposed to LETV or a closely related virus and that loggerhead turtles may be exposed as well. Based on a cutoff optical density value of 0.310, 71 out of the 329 wild Florida green turtles tested were seropositive for LETV antibodies (seroprevalence = 21.6%). In addition, no relationship between FP tumor severity or status and the presence of anti-LETV antibodies was found, further supporting the hypothesis that LETV and the FP-associated herpesvirus (FPHV) are separate infections of marine turtles.

KW - Antibodies

KW - Chelonia mydas

KW - Chelonian herpesviruses

KW - ELISA

KW - Green turtle

KW - LETD

KW - LETD-associated herpesvirus

KW - LETV

KW - Lung-eye-trachea disease

UR - http://www.scopus.com/inward/record.url?scp=0035814149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035814149&partnerID=8YFLogxK

M3 - Article

VL - 47

SP - 159

EP - 167

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 3

ER -