### Abstract

Twenty-four control cases of reactive follicular hyperplasia (RFH) and 28 cases of B-cell non-Hodgkin's malignant lymphoma (ML) were evaluated for surface immunoglobulin (sIg) light chain expression with flow cytometry. Because there was no significant difference between the distribution of the ML κ/λ ratios for which κ/λ >1 compared with those ML λ/κ ratios for which λ/κ >1 using the Kolmogorov-Smirnov statistic, the authors treated κ/λ data symetrically. By transforming all κ/λ ratios to maximum ratios, that is, R(max) = max (κ/λ, λ/κ), so that all resulting ratios wer at least one, the authors defined a new statistic for evaluating sIg light chain restriction. There was a highly significant difference for equality of distribution of the data using the Mann-Whitney test (P < 0.0001). By choosing cut-points based on the R(max) statistic, the authors were able to separate the RFH and ML cases with sensitivity = 0.96 ± 0.07 (95% confidence) and with specificity = 0.91 ± 0.11 (95% confidence). These point estimates of sensitivity and specificity appear to be promising for the clinical utility of the R(max) statistic, even for cases of B-cell ML with discordantly low expression of sIg light chains. The R(max) statistic will facilitate the comparison of data between different laboratories because it does not require assignment of cut-points and can be made using a simple nonparametric test.

Original language | English (US) |
---|---|

Pages (from-to) | 583-586 |

Number of pages | 4 |

Journal | American Journal of Clinical Pathology |

Volume | 91 |

Issue number | 5 |

State | Published - 1989 |

Externally published | Yes |

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### ASJC Scopus subject areas

- Pathology and Forensic Medicine

### Cite this

*American Journal of Clinical Pathology*,

*91*(5), 583-586.

**Surface immunoglobulin light chain restriction in B-cell non-Hodgkin's malignant lymphomas.** / Ratech, H.; Litwin, S.

Research output: Contribution to journal › Article

*American Journal of Clinical Pathology*, vol. 91, no. 5, pp. 583-586.

}

TY - JOUR

T1 - Surface immunoglobulin light chain restriction in B-cell non-Hodgkin's malignant lymphomas

AU - Ratech, H.

AU - Litwin, S.

PY - 1989

Y1 - 1989

N2 - Twenty-four control cases of reactive follicular hyperplasia (RFH) and 28 cases of B-cell non-Hodgkin's malignant lymphoma (ML) were evaluated for surface immunoglobulin (sIg) light chain expression with flow cytometry. Because there was no significant difference between the distribution of the ML κ/λ ratios for which κ/λ >1 compared with those ML λ/κ ratios for which λ/κ >1 using the Kolmogorov-Smirnov statistic, the authors treated κ/λ data symetrically. By transforming all κ/λ ratios to maximum ratios, that is, R(max) = max (κ/λ, λ/κ), so that all resulting ratios wer at least one, the authors defined a new statistic for evaluating sIg light chain restriction. There was a highly significant difference for equality of distribution of the data using the Mann-Whitney test (P < 0.0001). By choosing cut-points based on the R(max) statistic, the authors were able to separate the RFH and ML cases with sensitivity = 0.96 ± 0.07 (95% confidence) and with specificity = 0.91 ± 0.11 (95% confidence). These point estimates of sensitivity and specificity appear to be promising for the clinical utility of the R(max) statistic, even for cases of B-cell ML with discordantly low expression of sIg light chains. The R(max) statistic will facilitate the comparison of data between different laboratories because it does not require assignment of cut-points and can be made using a simple nonparametric test.

AB - Twenty-four control cases of reactive follicular hyperplasia (RFH) and 28 cases of B-cell non-Hodgkin's malignant lymphoma (ML) were evaluated for surface immunoglobulin (sIg) light chain expression with flow cytometry. Because there was no significant difference between the distribution of the ML κ/λ ratios for which κ/λ >1 compared with those ML λ/κ ratios for which λ/κ >1 using the Kolmogorov-Smirnov statistic, the authors treated κ/λ data symetrically. By transforming all κ/λ ratios to maximum ratios, that is, R(max) = max (κ/λ, λ/κ), so that all resulting ratios wer at least one, the authors defined a new statistic for evaluating sIg light chain restriction. There was a highly significant difference for equality of distribution of the data using the Mann-Whitney test (P < 0.0001). By choosing cut-points based on the R(max) statistic, the authors were able to separate the RFH and ML cases with sensitivity = 0.96 ± 0.07 (95% confidence) and with specificity = 0.91 ± 0.11 (95% confidence). These point estimates of sensitivity and specificity appear to be promising for the clinical utility of the R(max) statistic, even for cases of B-cell ML with discordantly low expression of sIg light chains. The R(max) statistic will facilitate the comparison of data between different laboratories because it does not require assignment of cut-points and can be made using a simple nonparametric test.

UR - http://www.scopus.com/inward/record.url?scp=0024550554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024550554&partnerID=8YFLogxK

M3 - Article

C2 - 2497637

AN - SCOPUS:0024550554

VL - 91

SP - 583

EP - 586

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 5

ER -