Suppression of human lymphocyte DNA and protein synthesis in vitro by adenosine and eight modified adenine nucleosides in the presence or in the absence of adenosine deaminase inhibitors, 2′-deoxycoformycin (DCF) and erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA)

Howard Ratech, Lloyd Kuritsky, G. Jeanette Thorbecke, Rochelle Hirschhorn

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

Adenosine and eight modified adenine nucleosides in the presence or in the absence of adenosine deaminase (ADA) inhibitors, 2′-deoxycoformycin or erythro-9-(2-hydroxy-3-nonyl) adenine, were investigated with regard to their effects on phytohemagglutinin-stimulated human lymphocytes in vitro. Protein and DNA synthesis were inhibited depending upon the specific nucleoside, presence or absence of ADA inhibitor, and time of addition of nucleoside to cultures. A possible role for the modified adenine nucleosides in causing in vivo immunodeficiency is proposed.

Original languageEnglish (US)
Pages (from-to)244-251
Number of pages8
JournalCellular Immunology
Volume68
Issue number2
DOIs
StatePublished - 1982
Externally publishedYes

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Adenosine Deaminase Inhibitors
Pentostatin
Adenine
Nucleosides
Adenosine
Lymphocytes
DNA
Proteins
Phytohemagglutinins
9-(2-hydroxy-3-nonyl)adenine
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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Suppression of human lymphocyte DNA and protein synthesis in vitro by adenosine and eight modified adenine nucleosides in the presence or in the absence of adenosine deaminase inhibitors, 2′-deoxycoformycin (DCF) and erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA). / Ratech, Howard; Kuritsky, Lloyd; Thorbecke, G. Jeanette; Hirschhorn, Rochelle.

In: Cellular Immunology, Vol. 68, No. 2, 1982, p. 244-251.

Research output: Contribution to journal › Article

Ratech, H, Kuritsky, L, Thorbecke, GJ & Hirschhorn, R 1982, 'Suppression of human lymphocyte DNA and protein synthesis in vitro by adenosine and eight modified adenine nucleosides in the presence or in the absence of adenosine deaminase inhibitors, 2′-deoxycoformycin (DCF) and erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA)', Cellular Immunology, vol. 68, no. 2, pp. 244-251. https://doi.org/10.1016/0008-8749(82)90109-5
Ratech H, Kuritsky L, Thorbecke GJ, Hirschhorn R. Suppression of human lymphocyte DNA and protein synthesis in vitro by adenosine and eight modified adenine nucleosides in the presence or in the absence of adenosine deaminase inhibitors, 2′-deoxycoformycin (DCF) and erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA). Cellular Immunology. 1982;68(2):244-251. https://doi.org/10.1016/0008-8749(82)90109-5
Ratech, Howard ; Kuritsky, Lloyd ; Thorbecke, G. Jeanette ; Hirschhorn, Rochelle. / Suppression of human lymphocyte DNA and protein synthesis in vitro by adenosine and eight modified adenine nucleosides in the presence or in the absence of adenosine deaminase inhibitors, 2′-deoxycoformycin (DCF) and erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA). In: Cellular Immunology. 1982 ; Vol. 68, No. 2. pp. 244-251.
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