Suppression of endogenous glucose production by isoleucine and valine and impact of diet composition

Isabel Arrieta-Cruz, Ya Su, Roger Gutiérrez-Juárez

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Leucine has been shown to acutely inhibit hepatic glucose production in rodents by a mechanism requiring its metabolism to acetyl-CoA in the mediobasal hypothalamus (MBH). In the early stages, all branched-chain amino acids (BCAA) are metabolized by a shared set of enzymes to produce a ketoacid, which is later metabolized to acetyl-CoA. Consequently, isoleucine and valine may also modulate glucose metabolism. To examine this possibility we performed intrahypothalamic infusions of isoleucine or valine in rats and assessed whole body glucose kinetics under basal conditions and during euglycemic pancreatic clamps. Furthermore, because high fat diet (HFD) consumption is known to interfere with central glucoregulation, we also asked whether the action of BCAAs was affected by HFD. We fed rats a lard-rich diet for a short interval and examined their response to central leucine. The results showed that both isoleucine and valine individually lowered blood glucose by decreasing liver glucose production. Furthermore, the action of the BCAA leucine was markedly attenuated by HFD feeding. We conclude that all three BCAAs centrally modulate glucose metabolism in the liver and that their action is disrupted by HFD-induced insulin resistance.

Original languageEnglish (US)
Article number79
JournalNutrients
Volume8
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Isoleucine
Valine
isoleucine
valine
High Fat Diet
high fat diet
Diet
Glucose
glucose
Leucine
leucine
Branched Chain Amino Acids
Acetyl Coenzyme A
diet
branched chain amino acids
liver
metabolism
glucoregulation
Liver
Glucose Clamp Technique

Keywords

  • Branched-chain amino acids
  • Glucose metabolism
  • Hypothalamus
  • Isoleucine
  • Leucine
  • Liver
  • Nutrient sensing
  • Valine

ASJC Scopus subject areas

  • Food Science

Cite this

Suppression of endogenous glucose production by isoleucine and valine and impact of diet composition. / Arrieta-Cruz, Isabel; Su, Ya; Gutiérrez-Juárez, Roger.

In: Nutrients, Vol. 8, No. 2, 79, 01.02.2016.

Research output: Contribution to journalArticle

Arrieta-Cruz, Isabel ; Su, Ya ; Gutiérrez-Juárez, Roger. / Suppression of endogenous glucose production by isoleucine and valine and impact of diet composition. In: Nutrients. 2016 ; Vol. 8, No. 2.
@article{851ff2a17b1a46e38b8a85d84fd0f431,
title = "Suppression of endogenous glucose production by isoleucine and valine and impact of diet composition",
abstract = "Leucine has been shown to acutely inhibit hepatic glucose production in rodents by a mechanism requiring its metabolism to acetyl-CoA in the mediobasal hypothalamus (MBH). In the early stages, all branched-chain amino acids (BCAA) are metabolized by a shared set of enzymes to produce a ketoacid, which is later metabolized to acetyl-CoA. Consequently, isoleucine and valine may also modulate glucose metabolism. To examine this possibility we performed intrahypothalamic infusions of isoleucine or valine in rats and assessed whole body glucose kinetics under basal conditions and during euglycemic pancreatic clamps. Furthermore, because high fat diet (HFD) consumption is known to interfere with central glucoregulation, we also asked whether the action of BCAAs was affected by HFD. We fed rats a lard-rich diet for a short interval and examined their response to central leucine. The results showed that both isoleucine and valine individually lowered blood glucose by decreasing liver glucose production. Furthermore, the action of the BCAA leucine was markedly attenuated by HFD feeding. We conclude that all three BCAAs centrally modulate glucose metabolism in the liver and that their action is disrupted by HFD-induced insulin resistance.",
keywords = "Branched-chain amino acids, Glucose metabolism, Hypothalamus, Isoleucine, Leucine, Liver, Nutrient sensing, Valine",
author = "Isabel Arrieta-Cruz and Ya Su and Roger Guti{\'e}rrez-Ju{\'a}rez",
year = "2016",
month = "2",
day = "1",
doi = "10.3390/nu8020079",
language = "English (US)",
volume = "8",
journal = "Nutrients",
issn = "2072-6643",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

TY - JOUR

T1 - Suppression of endogenous glucose production by isoleucine and valine and impact of diet composition

AU - Arrieta-Cruz, Isabel

AU - Su, Ya

AU - Gutiérrez-Juárez, Roger

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Leucine has been shown to acutely inhibit hepatic glucose production in rodents by a mechanism requiring its metabolism to acetyl-CoA in the mediobasal hypothalamus (MBH). In the early stages, all branched-chain amino acids (BCAA) are metabolized by a shared set of enzymes to produce a ketoacid, which is later metabolized to acetyl-CoA. Consequently, isoleucine and valine may also modulate glucose metabolism. To examine this possibility we performed intrahypothalamic infusions of isoleucine or valine in rats and assessed whole body glucose kinetics under basal conditions and during euglycemic pancreatic clamps. Furthermore, because high fat diet (HFD) consumption is known to interfere with central glucoregulation, we also asked whether the action of BCAAs was affected by HFD. We fed rats a lard-rich diet for a short interval and examined their response to central leucine. The results showed that both isoleucine and valine individually lowered blood glucose by decreasing liver glucose production. Furthermore, the action of the BCAA leucine was markedly attenuated by HFD feeding. We conclude that all three BCAAs centrally modulate glucose metabolism in the liver and that their action is disrupted by HFD-induced insulin resistance.

AB - Leucine has been shown to acutely inhibit hepatic glucose production in rodents by a mechanism requiring its metabolism to acetyl-CoA in the mediobasal hypothalamus (MBH). In the early stages, all branched-chain amino acids (BCAA) are metabolized by a shared set of enzymes to produce a ketoacid, which is later metabolized to acetyl-CoA. Consequently, isoleucine and valine may also modulate glucose metabolism. To examine this possibility we performed intrahypothalamic infusions of isoleucine or valine in rats and assessed whole body glucose kinetics under basal conditions and during euglycemic pancreatic clamps. Furthermore, because high fat diet (HFD) consumption is known to interfere with central glucoregulation, we also asked whether the action of BCAAs was affected by HFD. We fed rats a lard-rich diet for a short interval and examined their response to central leucine. The results showed that both isoleucine and valine individually lowered blood glucose by decreasing liver glucose production. Furthermore, the action of the BCAA leucine was markedly attenuated by HFD feeding. We conclude that all three BCAAs centrally modulate glucose metabolism in the liver and that their action is disrupted by HFD-induced insulin resistance.

KW - Branched-chain amino acids

KW - Glucose metabolism

KW - Hypothalamus

KW - Isoleucine

KW - Leucine

KW - Liver

KW - Nutrient sensing

KW - Valine

UR - http://www.scopus.com/inward/record.url?scp=84958538309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84958538309&partnerID=8YFLogxK

U2 - 10.3390/nu8020079

DO - 10.3390/nu8020079

M3 - Article

VL - 8

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 2

M1 - 79

ER -