Suppression of artefacts in nuclear overhauser effect difference spectroscopy by reference deconvolution

Gareth A. Morris, David Cowburn

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Reference deconvolution allows the effects of common instrumental imperfections in Fourier transform NMR, including field inhomogeneity, field/frequency ratio instability and pulse phase and amplitude irreproducibility, to be removed from spectra containing a well‐resolved reference signal. By applying reference deconvolution to the two component spectra, perturbed and unperturbed, of a nuclear Overhauser effect difference spectrum, the artefacts that instrumental imperfections normally cause in the difference spectrum may be suppressed. A small penalty in signal‐to‐noise ratio is paid for an improvement of several orders of magnitude in artefact suppression, allowing even very small Overhauser enhancements to be detected with confidence. Results are presented showing the reduction of difference artefacts for methyl singlets, normally the most severe test for NOE difference spectra, to below 1 part in 50 000.

Original languageEnglish (US)
Pages (from-to)1085-1089
Number of pages5
JournalMagnetic Resonance in Chemistry
Volume27
Issue number11
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Deconvolution
Spectroscopy
Defects
Fourier transforms
Nuclear magnetic resonance

Keywords

  • H NMR
  • Artefacts
  • Difference spectroscopy
  • Nuclear Overhauser effect
  • Reference deconvolution

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)

Cite this

Suppression of artefacts in nuclear overhauser effect difference spectroscopy by reference deconvolution. / Morris, Gareth A.; Cowburn, David.

In: Magnetic Resonance in Chemistry, Vol. 27, No. 11, 1989, p. 1085-1089.

Research output: Contribution to journalArticle

@article{c1083c72ff9e453a9de32b48be00d93b,
title = "Suppression of artefacts in nuclear overhauser effect difference spectroscopy by reference deconvolution",
abstract = "Reference deconvolution allows the effects of common instrumental imperfections in Fourier transform NMR, including field inhomogeneity, field/frequency ratio instability and pulse phase and amplitude irreproducibility, to be removed from spectra containing a well‐resolved reference signal. By applying reference deconvolution to the two component spectra, perturbed and unperturbed, of a nuclear Overhauser effect difference spectrum, the artefacts that instrumental imperfections normally cause in the difference spectrum may be suppressed. A small penalty in signal‐to‐noise ratio is paid for an improvement of several orders of magnitude in artefact suppression, allowing even very small Overhauser enhancements to be detected with confidence. Results are presented showing the reduction of difference artefacts for methyl singlets, normally the most severe test for NOE difference spectra, to below 1 part in 50 000.",
keywords = "H NMR, Artefacts, Difference spectroscopy, Nuclear Overhauser effect, Reference deconvolution",
author = "Morris, {Gareth A.} and David Cowburn",
year = "1989",
doi = "10.1002/mrc.1260271112",
language = "English (US)",
volume = "27",
pages = "1085--1089",
journal = "Magnetic Resonance in Chemistry",
issn = "0749-1581",
publisher = "John Wiley and Sons Ltd",
number = "11",

}

TY - JOUR

T1 - Suppression of artefacts in nuclear overhauser effect difference spectroscopy by reference deconvolution

AU - Morris, Gareth A.

AU - Cowburn, David

PY - 1989

Y1 - 1989

N2 - Reference deconvolution allows the effects of common instrumental imperfections in Fourier transform NMR, including field inhomogeneity, field/frequency ratio instability and pulse phase and amplitude irreproducibility, to be removed from spectra containing a well‐resolved reference signal. By applying reference deconvolution to the two component spectra, perturbed and unperturbed, of a nuclear Overhauser effect difference spectrum, the artefacts that instrumental imperfections normally cause in the difference spectrum may be suppressed. A small penalty in signal‐to‐noise ratio is paid for an improvement of several orders of magnitude in artefact suppression, allowing even very small Overhauser enhancements to be detected with confidence. Results are presented showing the reduction of difference artefacts for methyl singlets, normally the most severe test for NOE difference spectra, to below 1 part in 50 000.

AB - Reference deconvolution allows the effects of common instrumental imperfections in Fourier transform NMR, including field inhomogeneity, field/frequency ratio instability and pulse phase and amplitude irreproducibility, to be removed from spectra containing a well‐resolved reference signal. By applying reference deconvolution to the two component spectra, perturbed and unperturbed, of a nuclear Overhauser effect difference spectrum, the artefacts that instrumental imperfections normally cause in the difference spectrum may be suppressed. A small penalty in signal‐to‐noise ratio is paid for an improvement of several orders of magnitude in artefact suppression, allowing even very small Overhauser enhancements to be detected with confidence. Results are presented showing the reduction of difference artefacts for methyl singlets, normally the most severe test for NOE difference spectra, to below 1 part in 50 000.

KW - H NMR

KW - Artefacts

KW - Difference spectroscopy

KW - Nuclear Overhauser effect

KW - Reference deconvolution

UR - http://www.scopus.com/inward/record.url?scp=84989078289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989078289&partnerID=8YFLogxK

U2 - 10.1002/mrc.1260271112

DO - 10.1002/mrc.1260271112

M3 - Article

AN - SCOPUS:84989078289

VL - 27

SP - 1085

EP - 1089

JO - Magnetic Resonance in Chemistry

JF - Magnetic Resonance in Chemistry

SN - 0749-1581

IS - 11

ER -