TY - JOUR
T1 - Superantigen modulation of experimental allergic encephalomyelitis
T2 - Activation or anergy determines outcome
AU - Racke, Michael K.
AU - Quigley, Laura
AU - Cannella, Barbara
AU - Raine, Cedric S.
AU - McFarlin, Dale E.
AU - Scott, Dorothy E.
PY - 1994/2/15
Y1 - 1994/2/15
N2 - Experimental allergic encephalomyelitis (EAE) is an autoimmune disease that can be induced by the adoptive transfer of CD4, myelin basic protein (MBP)-specific T cells. Superantigens activate T cells expressing appropriate TCR V genes. In this study, MBP-specific T cells activated in vitro with a superantigen, staphylococcal enterotoxin B (SEB), could adoptively transfer a severe form of EAE in (PLxSJL)F1 mice, but did not transfer disease in PL/J or SJL/J mice. SEB treatment of donor mice anergized MBP-specific T cells using Vβ8 in (PLxSJL)F1 mice, because subsequent in vitro activation with SFB resulted in a marked decrease in proliferation to SEB and inability to transfer EAE. However, donor cells from (PLxSJL)F1 mice immunized with MBP/CFA that had been exposed to SEB in vivo before MBP stimulation in vitro still produced EAE in recipient mice. To confirm that non-Vβ8 T cells could transfer disease, donor mice were treated with antibody that eliminated Vβ8 T cells; MBP-activated T cells from these mice could still transfer EAE. Finally, EAE induced by SEB-activated T cells was substantially reduced in mice receiving anti-Vβ8 therapy in vivo. The ability of superantigens to activate encephalitogenic T cells may have relevance to human diseases such as multiple sclerosis.
AB - Experimental allergic encephalomyelitis (EAE) is an autoimmune disease that can be induced by the adoptive transfer of CD4, myelin basic protein (MBP)-specific T cells. Superantigens activate T cells expressing appropriate TCR V genes. In this study, MBP-specific T cells activated in vitro with a superantigen, staphylococcal enterotoxin B (SEB), could adoptively transfer a severe form of EAE in (PLxSJL)F1 mice, but did not transfer disease in PL/J or SJL/J mice. SEB treatment of donor mice anergized MBP-specific T cells using Vβ8 in (PLxSJL)F1 mice, because subsequent in vitro activation with SFB resulted in a marked decrease in proliferation to SEB and inability to transfer EAE. However, donor cells from (PLxSJL)F1 mice immunized with MBP/CFA that had been exposed to SEB in vivo before MBP stimulation in vitro still produced EAE in recipient mice. To confirm that non-Vβ8 T cells could transfer disease, donor mice were treated with antibody that eliminated Vβ8 T cells; MBP-activated T cells from these mice could still transfer EAE. Finally, EAE induced by SEB-activated T cells was substantially reduced in mice receiving anti-Vβ8 therapy in vivo. The ability of superantigens to activate encephalitogenic T cells may have relevance to human diseases such as multiple sclerosis.
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M3 - Article
C2 - 8120406
AN - SCOPUS:0028296055
SN - 0022-1767
VL - 152
SP - 2051
EP - 2059
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -