Marcaje de leucocitos con 99mTc-HMPAO y 99mTc-gentisico empleando volumenes sanguineos pequenos

Translated title of the contribution: 99mTc-gentisic acid or 99mTc-HMPAO-labelling of leukocytes employing small blood volumes

M. A. Gonzalez Zavala, G. Ferro Flores, E. Avila Ramirez, P. Arzate Barbosa, R. J. Hernandez Valencia, G. Cardenas Marcial, R. Garcia Cortes

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A simplified procedure for 99mTc-gentisic acid or 99mTc-HMPAO-labelling of leukocytes in pediatric patients has been developed. For this purpose leukocytes were isolated from 3-10 ml. of whole blood and labelled, washed and resuspended in saline isotonic solution avoiding vial replacement. Validation of the technique was carried out with respect to 'in vitro' cell viability, sterility, phagocytic and chemotactic functions which remained unaffected. Labelling efficiencies employing 3 ml. of whole blood were 51 ± 8% and 47 ± 5% for 99mTc-HMPAO and 99mTc-gentisic acid respectively. The procedure described herein was used successfully in our clinic for scintigraphic localization of inflammatory lesions and abscesses in children.

Translated title of the contribution99mTc-gentisic acid or 99mTc-HMPAO-labelling of leukocytes employing small blood volumes
Original languageSpanish
Pages (from-to)244-249
Number of pages6
JournalRevista Espanola de Medicina Nuclear
Volume15
Issue number4
StatePublished - 1996
Externally publishedYes

Keywords

  • Tc-gentisic acid
  • Tc-HMPAO
  • radiolabelling of leukocytes
  • scintigraphic localization of abscesses
  • viability, phagocytic and chemotactic functions

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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