SUN2 overexpression deforms nuclear shape and inhibits HIV

Daniel A. Donahue, Sonia Amraoui, Francesca di Nunzio, Camille Kieffer, Françoise Porrot, Silvana Opp, Felipe Diaz-Griffero, Nicoletta Casartelli, Olivier Schwartz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In a previous screen of putative interferon-stimulated genes, SUN2 was shown to inhibit HIV-1 infection in an uncharacterized manner. SUN2 is an inner nuclear membrane protein belonging to the linker of nucleoskeleton and cytoskeleton complex. We have analyzed here the role of SUN2 in HIV infection. We report that in contrast to what was initially thought, SUN2 is not induced by type I interferon, and that SUN2 silencing does not modulate HIV infection. However, SUN2 overexpression in cell lines and in primary monocyte-derived dendritic cells inhibits the replication of HIV but not murine leukemia virus or chikungunya virus. We identified HIV-1 and HIV-2 strains that are unaffected by SUN2, suggesting that the effect is specific to particular viral components or cofactors. Intriguingly, SUN2 overexpression induces a multilobular flower-like nuclear shape that does not impact cell viability and is similar to that of cells isolated from patients with HTLV-I-associated adult T-cell leukemia or with progeria. Nuclear shape changes and HIV inhibition both mapped to the nucleoplasmic domain of SUN2 that interacts with the nuclear lamina. This block to HIV replication occurs between reverse transcription and nuclear entry, and passaging experiments selected for a single-amino-acid change in capsid (CA) that leads to resistance to overexpressed SUN2. Furthermore, using chemical inhibition or silencing of cyclophilin A (CypA), as well as CA mutant viruses, we implicated CypA in the SUN2-imposed block to HIV infection. Our results demonstrate that SUN2 overexpression perturbs both nuclear shape and early events of HIV infection.

Original languageEnglish (US)
Pages (from-to)4199-4214
Number of pages16
JournalJournal of Virology
Volume90
Issue number8
DOIs
StatePublished - Apr 1 2016

Fingerprint

HIV infections
HIV Infections
HIV
cyclophilins
capsid
Cyclophilin A
interferons
Human immunodeficiency virus 1
Capsid
Chikungunya virus
Human immunodeficiency virus 2
Murine leukemia virus
HIV-1
reverse transcription
Progeria
Nuclear Lamina
nuclear proteins
nuclear membrane
dendritic cells
Nuclear Matrix

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Donahue, D. A., Amraoui, S., di Nunzio, F., Kieffer, C., Porrot, F., Opp, S., ... Schwartz, O. (2016). SUN2 overexpression deforms nuclear shape and inhibits HIV. Journal of Virology, 90(8), 4199-4214. https://doi.org/10.1128/JVI.03202-15

SUN2 overexpression deforms nuclear shape and inhibits HIV. / Donahue, Daniel A.; Amraoui, Sonia; di Nunzio, Francesca; Kieffer, Camille; Porrot, Françoise; Opp, Silvana; Diaz-Griffero, Felipe; Casartelli, Nicoletta; Schwartz, Olivier.

In: Journal of Virology, Vol. 90, No. 8, 01.04.2016, p. 4199-4214.

Research output: Contribution to journalArticle

Donahue, DA, Amraoui, S, di Nunzio, F, Kieffer, C, Porrot, F, Opp, S, Diaz-Griffero, F, Casartelli, N & Schwartz, O 2016, 'SUN2 overexpression deforms nuclear shape and inhibits HIV', Journal of Virology, vol. 90, no. 8, pp. 4199-4214. https://doi.org/10.1128/JVI.03202-15
Donahue DA, Amraoui S, di Nunzio F, Kieffer C, Porrot F, Opp S et al. SUN2 overexpression deforms nuclear shape and inhibits HIV. Journal of Virology. 2016 Apr 1;90(8):4199-4214. https://doi.org/10.1128/JVI.03202-15
Donahue, Daniel A. ; Amraoui, Sonia ; di Nunzio, Francesca ; Kieffer, Camille ; Porrot, Françoise ; Opp, Silvana ; Diaz-Griffero, Felipe ; Casartelli, Nicoletta ; Schwartz, Olivier. / SUN2 overexpression deforms nuclear shape and inhibits HIV. In: Journal of Virology. 2016 ; Vol. 90, No. 8. pp. 4199-4214.
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