Success of Murine embryo transfer increased by a modified transfer pipette

Zheng Zhang, L. V. Xiaoyan, Yidong Wang, Ye Chen, Rong Zheng, Huan Sun, Guohui Bian, Yan Xiao, Qingwei Li, Qiutan Yang, Jianzhong Ai, Jingjing Duan, Ruizhi Tan, Yuhang Liu, Yang Yang, Yuquan Wei, Qin Zhou

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Embryos transfer is one of the most critical steps for generating genetically modified mice. Because of the limitations of the current transfer equipment and techniques, such as discharge of freshly transferred embryos and the necessity of sophisticated transfer skills, the success ratio of implantation may not well satisfy the requirements for mass production of high quality animals in the field of genetically modified mice. In this study, we describe a modified embryo transfer pipette with a syringe-like tip that can easily be applied to mouse embryo transfer. Using this improved method, we show that the procedures for mouse embryo implantation are simplified and easier to perform; moreover, the viability rate of mouse embryos is 20% higher than that achieved with conventional methods. Our modified tool and improved transfer technique are effective, time-saving and less invasive, resulting in increased success of embryo transfer.

Original languageEnglish (US)
Pages (from-to)94-97
Number of pages4
JournalJournal of Reproduction and Development
Volume55
Issue number1
DOIs
Publication statusPublished - Feb 1 2009

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Keywords

  • Embryo transfer
  • Implantation
  • Mouse
  • Oviduct
  • Reproduction
  • Uterus

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Zhang, Z., Xiaoyan, L. V., Wang, Y., Chen, Y., Zheng, R., Sun, H., ... Zhou, Q. (2009). Success of Murine embryo transfer increased by a modified transfer pipette. Journal of Reproduction and Development, 55(1), 94-97. https://doi.org/10.1262/jrd.20090