Success of Murine embryo transfer increased by a modified transfer pipette

Zheng Zhang, L. V. Xiaoyan, Yidong Wang, Ye Chen, Rong Zheng, Huan Sun, Guohui Bian, Yan Xiao, Qingwei Li, Qiutan Yang, Jianzhong Ai, Jingjing Duan, Ruizhi Tan, Yuhang Liu, Yang Yang, Yuquan Wei, Qin Zhou

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Embryos transfer is one of the most critical steps for generating genetically modified mice. Because of the limitations of the current transfer equipment and techniques, such as discharge of freshly transferred embryos and the necessity of sophisticated transfer skills, the success ratio of implantation may not well satisfy the requirements for mass production of high quality animals in the field of genetically modified mice. In this study, we describe a modified embryo transfer pipette with a syringe-like tip that can easily be applied to mouse embryo transfer. Using this improved method, we show that the procedures for mouse embryo implantation are simplified and easier to perform; moreover, the viability rate of mouse embryos is 20% higher than that achieved with conventional methods. Our modified tool and improved transfer technique are effective, time-saving and less invasive, resulting in increased success of embryo transfer.

Original languageEnglish (US)
Pages (from-to)94-97
Number of pages4
JournalJournal of Reproduction and Development
Volume55
Issue number1
DOIs
StatePublished - Feb 2009
Externally publishedYes

Fingerprint

embryo transfer
mice
embryo (animal)
embryo implantation
syringes
methodology
viability
animals

Keywords

  • Embryo transfer
  • Implantation
  • Mouse
  • Oviduct
  • Reproduction
  • Uterus

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Success of Murine embryo transfer increased by a modified transfer pipette. / Zhang, Zheng; Xiaoyan, L. V.; Wang, Yidong; Chen, Ye; Zheng, Rong; Sun, Huan; Bian, Guohui; Xiao, Yan; Li, Qingwei; Yang, Qiutan; Ai, Jianzhong; Duan, Jingjing; Tan, Ruizhi; Liu, Yuhang; Yang, Yang; Wei, Yuquan; Zhou, Qin.

In: Journal of Reproduction and Development, Vol. 55, No. 1, 02.2009, p. 94-97.

Research output: Contribution to journalArticle

Zhang, Z, Xiaoyan, LV, Wang, Y, Chen, Y, Zheng, R, Sun, H, Bian, G, Xiao, Y, Li, Q, Yang, Q, Ai, J, Duan, J, Tan, R, Liu, Y, Yang, Y, Wei, Y & Zhou, Q 2009, 'Success of Murine embryo transfer increased by a modified transfer pipette', Journal of Reproduction and Development, vol. 55, no. 1, pp. 94-97. https://doi.org/10.1262/jrd.20090
Zhang, Zheng ; Xiaoyan, L. V. ; Wang, Yidong ; Chen, Ye ; Zheng, Rong ; Sun, Huan ; Bian, Guohui ; Xiao, Yan ; Li, Qingwei ; Yang, Qiutan ; Ai, Jianzhong ; Duan, Jingjing ; Tan, Ruizhi ; Liu, Yuhang ; Yang, Yang ; Wei, Yuquan ; Zhou, Qin. / Success of Murine embryo transfer increased by a modified transfer pipette. In: Journal of Reproduction and Development. 2009 ; Vol. 55, No. 1. pp. 94-97.
@article{a5999c6abedd457b8e2ecf1db90987ba,
title = "Success of Murine embryo transfer increased by a modified transfer pipette",
abstract = "Embryos transfer is one of the most critical steps for generating genetically modified mice. Because of the limitations of the current transfer equipment and techniques, such as discharge of freshly transferred embryos and the necessity of sophisticated transfer skills, the success ratio of implantation may not well satisfy the requirements for mass production of high quality animals in the field of genetically modified mice. In this study, we describe a modified embryo transfer pipette with a syringe-like tip that can easily be applied to mouse embryo transfer. Using this improved method, we show that the procedures for mouse embryo implantation are simplified and easier to perform; moreover, the viability rate of mouse embryos is 20{\%} higher than that achieved with conventional methods. Our modified tool and improved transfer technique are effective, time-saving and less invasive, resulting in increased success of embryo transfer.",
keywords = "Embryo transfer, Implantation, Mouse, Oviduct, Reproduction, Uterus",
author = "Zheng Zhang and Xiaoyan, {L. V.} and Yidong Wang and Ye Chen and Rong Zheng and Huan Sun and Guohui Bian and Yan Xiao and Qingwei Li and Qiutan Yang and Jianzhong Ai and Jingjing Duan and Ruizhi Tan and Yuhang Liu and Yang Yang and Yuquan Wei and Qin Zhou",
year = "2009",
month = "2",
doi = "10.1262/jrd.20090",
language = "English (US)",
volume = "55",
pages = "94--97",
journal = "Journal of Reproduction and Development",
issn = "0916-8818",
publisher = "Japanese Society of Animal Reproduction (JSAR)",
number = "1",

}

TY - JOUR

T1 - Success of Murine embryo transfer increased by a modified transfer pipette

AU - Zhang, Zheng

AU - Xiaoyan, L. V.

AU - Wang, Yidong

AU - Chen, Ye

AU - Zheng, Rong

AU - Sun, Huan

AU - Bian, Guohui

AU - Xiao, Yan

AU - Li, Qingwei

AU - Yang, Qiutan

AU - Ai, Jianzhong

AU - Duan, Jingjing

AU - Tan, Ruizhi

AU - Liu, Yuhang

AU - Yang, Yang

AU - Wei, Yuquan

AU - Zhou, Qin

PY - 2009/2

Y1 - 2009/2

N2 - Embryos transfer is one of the most critical steps for generating genetically modified mice. Because of the limitations of the current transfer equipment and techniques, such as discharge of freshly transferred embryos and the necessity of sophisticated transfer skills, the success ratio of implantation may not well satisfy the requirements for mass production of high quality animals in the field of genetically modified mice. In this study, we describe a modified embryo transfer pipette with a syringe-like tip that can easily be applied to mouse embryo transfer. Using this improved method, we show that the procedures for mouse embryo implantation are simplified and easier to perform; moreover, the viability rate of mouse embryos is 20% higher than that achieved with conventional methods. Our modified tool and improved transfer technique are effective, time-saving and less invasive, resulting in increased success of embryo transfer.

AB - Embryos transfer is one of the most critical steps for generating genetically modified mice. Because of the limitations of the current transfer equipment and techniques, such as discharge of freshly transferred embryos and the necessity of sophisticated transfer skills, the success ratio of implantation may not well satisfy the requirements for mass production of high quality animals in the field of genetically modified mice. In this study, we describe a modified embryo transfer pipette with a syringe-like tip that can easily be applied to mouse embryo transfer. Using this improved method, we show that the procedures for mouse embryo implantation are simplified and easier to perform; moreover, the viability rate of mouse embryos is 20% higher than that achieved with conventional methods. Our modified tool and improved transfer technique are effective, time-saving and less invasive, resulting in increased success of embryo transfer.

KW - Embryo transfer

KW - Implantation

KW - Mouse

KW - Oviduct

KW - Reproduction

KW - Uterus

UR - http://www.scopus.com/inward/record.url?scp=65349119596&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65349119596&partnerID=8YFLogxK

U2 - 10.1262/jrd.20090

DO - 10.1262/jrd.20090

M3 - Article

VL - 55

SP - 94

EP - 97

JO - Journal of Reproduction and Development

JF - Journal of Reproduction and Development

SN - 0916-8818

IS - 1

ER -