Subunit structure and amino acid composition of xylose isomerase from Streptomyces albus

R. A. Hogue Angeletti

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The subunit structure and amino acid composition of xylose isomerase from S. albus have been examined. A native molecular weight of 165,000 determined by sedimentation equilibrium was reduced to 43,000 when the protein was treated with 6 M guanidine hydrochloride. No further reduction in molecular weight was observed when potential disulfide bridges of xylose isomerase were reduced and alkylated, indicating that the protein was devoid of interchain disulfide bonds. NH2 terminal analysis using [3H]dansyl chloride showed 0.86 residues of methionine per M(r) = 41,500 unit. Analysis of the native protein with an automated protein sequenator revealed the presence of only one degradable polypeptide chain. Fractionation of the soluble tryptic peptides of S [14C]carboxymethyl xylose isomerase by ion exchange chromatography and one dimensional paper electrophoresis yielded 37 to 43 peptides. When the acid insoluble tryptic peptides were dissolved and analyzed using gel filtration techniques, an additional four peptides were found. A unique radioactive tryptic peptide containing S carboxymethylcysteine was found among the soluble peptides, confirming cysteine as the limiting amino acid residue in the amino acid composition of xylose isomerase. On the basis of its lysine and arginine content, the number of tryptic peptides is consistent with the hypothesis that the native xylose isomerase is a tetramer of four very similar or identical subunits of M(r) = 41,500 associated by noncovalent bonds.

Original languageEnglish (US)
Pages (from-to)7814-7818
Number of pages5
JournalJournal of Biological Chemistry
Volume250
Issue number19
StatePublished - 1975
Externally publishedYes

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xylose isomerase
Streptomyces
Amino Acids
Peptides
Chemical analysis
Disulfides
Proteins
Carbocysteine
Molecular Weight
Molecular weight
Paper Electrophoresis
Guanidine
Ion Exchange Chromatography
Fractionation
Chromatography
Electrophoresis
Sedimentation
Methionine
Lysine
Gel Chromatography

ASJC Scopus subject areas

  • Biochemistry

Cite this

Subunit structure and amino acid composition of xylose isomerase from Streptomyces albus. / Hogue Angeletti, R. A.

In: Journal of Biological Chemistry, Vol. 250, No. 19, 1975, p. 7814-7818.

Research output: Contribution to journalArticle

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