Substrate structural requirements of Schizosaccharomyces pombe RNase P

Denis Drainas; Steven Zimmerly; Ian Willis; Dieter Söll

doi:10.1016/0014-5793(89)81433-4

Substrate structural requirements of Schizosaccharomyces pombe RNase P

Denis Drainas, Steven Zimmerly, Ian Willis, Dieter Söll

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent K_m for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNA^Ser genes is 20 nM; the apparent V_max is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.

Original language	English (US)
Pages (from-to)	84-88
Number of pages	5
Journal	FEBS Letters
Volume	251
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(89)81433-4
State	Published - Jul 17 1989
Externally published	Yes

Keywords

(Schizosaccharomyces pombe)
RNase P
Substrate recognition
tRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(89)81433-4

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title = "Substrate structural requirements of Schizosaccharomyces pombe RNase P",

abstract = "RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent Km for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNASer genes is 20 nM; the apparent Vmax is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.",

keywords = "(Schizosaccharomyces pombe), RNase P, Substrate recognition, tRNA",

author = "Denis Drainas and Steven Zimmerly and Ian Willis and Dieter S{\"o}ll",

note = "Funding Information: AcknowledgementWs:e would like to thank ShobhaC har for adviceo n enzymep urificationa nd Astrid Schon for numerous helpful discussionsT. his work was supportedb y a grant from the National Instituteso f Health.",

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doi = "10.1016/0014-5793(89)81433-4",

language = "English (US)",

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T1 - Substrate structural requirements of Schizosaccharomyces pombe RNase P

AU - Drainas, Denis

AU - Zimmerly, Steven

AU - Willis, Ian

AU - Söll, Dieter

N1 - Funding Information: AcknowledgementWs:e would like to thank ShobhaC har for adviceo n enzymep urificationa nd Astrid Schon for numerous helpful discussionsT. his work was supportedb y a grant from the National Instituteso f Health.

PY - 1989/7/17

Y1 - 1989/7/17

N2 - RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent Km for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNASer genes is 20 nM; the apparent Vmax is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.

AB - RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent Km for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNASer genes is 20 nM; the apparent Vmax is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.

KW - (Schizosaccharomyces pombe)

KW - RNase P

KW - Substrate recognition

KW - tRNA

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Substrate structural requirements of Schizosaccharomyces pombe RNase P

Abstract

Keywords

ASJC Scopus subject areas

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