Substrate structural requirements of Schizosaccharomyces pombe RNase P

Denis Drainas, Steven Zimmerly, Ian Willis, Dieter Söll

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent Km for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNASer genes is 20 nM; the apparent Vmax is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.

Original languageEnglish (US)
Pages (from-to)84-88
Number of pages5
JournalFEBS Letters
Volume251
Issue number1-2
DOIs
StatePublished - Jul 17 1989
Externally publishedYes

Keywords

  • (Schizosaccharomyces pombe)
  • RNase P
  • Substrate recognition
  • tRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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