Substitutions that lock and unlock the proton-coupled folate transporter (PCFT-SLC46A1) in an inward-open conformation

Srinivas Aluri, Rongbao Zhao, Kai Lin, Daniel Sanghoon Shin, Andras Fiser, I. David Goldman

Research output: Contribution to journalArticle

Abstract

The proton-coupled folate transporter (PCFT) mediates intestinal absorption of folates and their transport from blood to cerebrospinal fluid across the choroid plexus. Substitutions at Asp-109 in the first intracellular loop between the first and second transmembrane domains (TMDs) abolish PCFT function, but protein expression and trafficking to the cell membrane are retained. Here, we used site-directed mutagenesis, the substituted- cysteine accessibility method, functional analyses,andhomology modeling to determine whether the D109A substitution locks PCFT in one of its conformational states. Cys-substituted residues lining the PCFT aqueous translocation pathway and accessible in WT PCFT to the membrane-impermeable cysteine- biotinylation reagent, MTSEA-biotin, lost accessibility when introduced into the D109A scaffold. Substitutions at Gly- 305 located exofacially within the eighthTMD, particularly with bulky residues, when introduced into the D109A scaffold largely restored function and MTSEA-biotin accessibility to Cys-substituted residues within the pathway. Likewise, Ser-196 substitution in the fifth TMD, predicted by homology modeling to be in proximity to Gly-305, also partially restored function found in solute transporters, is critical to oscillation of the carrier among its conformational states. Substitutions at Asp-109 and Gly-112 lock PCFT in an inward-open conformation, resulting in the loss of function. However, the integrity of the locked protein is preserved, indicated by the restoration of function after insertion of a second "unlocking" mutation. and accessibility. Similarly, the inactivating G112K substitution within the first intracellular loop was partially reactivated by introducing the G305L substitution. These data indicate that the first intracellular loop, with a sequence identical to "motif A" (GXXXDXXGR(R/K)).

Original languageEnglish (US)
Pages (from-to)7245-7258
Number of pages14
JournalJournal of Biological Chemistry
Volume294
Issue number18
DOIs
StatePublished - Jan 1 2019

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Proton-Coupled Folate Transporter
Conformations
Substitution reactions
Cysteine
Scaffolds
Biotinylation
Choroid Plexus
Cerebrospinal fluid
Intestinal Absorption
Protein Transport
Mutagenesis
Site-Directed Mutagenesis
Folic Acid
Cell membranes
Cerebrospinal Fluid
Linings
Restoration
Cell Membrane
Proteins
Blood

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Substitutions that lock and unlock the proton-coupled folate transporter (PCFT-SLC46A1) in an inward-open conformation. / Aluri, Srinivas; Zhao, Rongbao; Lin, Kai; Shin, Daniel Sanghoon; Fiser, Andras; Goldman, I. David.

In: Journal of Biological Chemistry, Vol. 294, No. 18, 01.01.2019, p. 7245-7258.

Research output: Contribution to journalArticle

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abstract = "The proton-coupled folate transporter (PCFT) mediates intestinal absorption of folates and their transport from blood to cerebrospinal fluid across the choroid plexus. Substitutions at Asp-109 in the first intracellular loop between the first and second transmembrane domains (TMDs) abolish PCFT function, but protein expression and trafficking to the cell membrane are retained. Here, we used site-directed mutagenesis, the substituted- cysteine accessibility method, functional analyses,andhomology modeling to determine whether the D109A substitution locks PCFT in one of its conformational states. Cys-substituted residues lining the PCFT aqueous translocation pathway and accessible in WT PCFT to the membrane-impermeable cysteine- biotinylation reagent, MTSEA-biotin, lost accessibility when introduced into the D109A scaffold. Substitutions at Gly- 305 located exofacially within the eighthTMD, particularly with bulky residues, when introduced into the D109A scaffold largely restored function and MTSEA-biotin accessibility to Cys-substituted residues within the pathway. Likewise, Ser-196 substitution in the fifth TMD, predicted by homology modeling to be in proximity to Gly-305, also partially restored function found in solute transporters, is critical to oscillation of the carrier among its conformational states. Substitutions at Asp-109 and Gly-112 lock PCFT in an inward-open conformation, resulting in the loss of function. However, the integrity of the locked protein is preserved, indicated by the restoration of function after insertion of a second {"}unlocking{"} mutation. and accessibility. Similarly, the inactivating G112K substitution within the first intracellular loop was partially reactivated by introducing the G305L substitution. These data indicate that the first intracellular loop, with a sequence identical to {"}motif A{"} (GXXXDXXGR(R/K)).",
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