Sub1 functions in osmoregulation and in transcription by both RNA polymerases II and III

Emanuel Rosonina, Ian M. Willis, James L. Manley

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Subl is implicated in transcriptional activation, elongation, and mRNA 3'-end formation in budding yeast. To gain more insight into its function, we performed a synthetic genetic array screen with SUB1 that uncovered genetic interactions with genes involved in the high-osmolarity glycerol (HOG) osmoresponse pathway. We find that Subl and the HOG pathway are redundant for survival in moderate osmolarity. Chromatin immunoprecipitation analysis shows that Subl is recruited to osmoresponse gene promoters during osmotic shock and is required for full recruitment of TBP, TFIIB, and RNA polymerase II (RNAP II) at a subset of these genes. Furthermore, we detect Subl at the promoter of every constitutively transcribed RNAP II and, unexpectedly, at every RNAP III gene tested, but not at the RNAP I-transcribed ribosomal DNA promoter. Significantly, deletion of SUB1 reduced levels of promoter-associated RNAP II or III at these genes, but not TBP levels. Together these data suggest that, in addition to a general role in polymerase recruitment at constitutive RNAP II and RNAP III genes, during osmotic shock, Subl facilitates osmoresponse gene transcription by enhancing preinitiation complex formation.

Original languageEnglish (US)
Pages (from-to)2307-2321
Number of pages15
JournalMolecular and Cellular Biology
Volume29
Issue number8
DOIs
StatePublished - Apr 2009

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RNA Polymerase III
Osmoregulation
RNA Polymerase II
Genes
Osmolar Concentration
Osmotic Pressure
Glycerol
Transcription Factor TFIIB
Saccharomycetales
Chromatin Immunoprecipitation
Ribosomal DNA
Transcriptional Activation
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Sub1 functions in osmoregulation and in transcription by both RNA polymerases II and III. / Rosonina, Emanuel; Willis, Ian M.; Manley, James L.

In: Molecular and Cellular Biology, Vol. 29, No. 8, 04.2009, p. 2307-2321.

Research output: Contribution to journalArticle

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