Study of the urinary metabolite profile associated with osteoarthritis and the correlation with its TCM syndromes

Li Xi Chu, Song Bin Yang, Wei Jia, Yunping Qiu, Ming Ming Su

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

To compare the distinction of endogenous small molecular metabolites in urine samples from the patients suffering from osteoarthritis(OA) and the healthy people. To contrast the variance of metabolic phenotypes belong to the two different TCM syndromes of OA. Methods: Urine samples of 85 participants (43 non-OA controls and 42 individuals with the disturbance of OA which include two different classes in TCM theory) were selected. Utilizing the combined gas chromatography and mass spectrometry (GC-MS) method for the detection of such urine samples. Pattern recognition was applied to analyze the raw data. Some potential biomarkers of OA were identified by means of retrieval of mass spectral database. By the same means, comparative study was carried out between the two groups of TCM syndromes. Results: Obvious differences were found in the total ion chromatograms (TIC) of urine samples between the OA and control subjects. When compared the insufficiency of liver and kidney with tendon and vessel stagnation syndrome group with the control, the plot of PCA scores showed that satisfactory separation was made. Better division was realized when the more sophisticated model PLSDA and OPLSDA were utilized. Well-pleasing separation was achieved when the comparison between of the asthenia of both spleen and kidney with dampness invading joints syndrome group and the control one as well as between of the two TCM syndromes. Conclusion: There are differences in the urine metabolite profiles among different types of syndrome of OA and the control group. The metabolic regulatory network in organism probably is perturbed in the progress of OA. The nature of types of syndrome of TCM maybe base on the variation of metabolites in vivo and the technique of metabonomics could facilitate the research of the nature of TCM theory.

Original languageEnglish (US)
Title of host publicationIFMBE Proceedings
Pages691-696
Number of pages6
Volume19 IFMBE
DOIs
StatePublished - 2008
Externally publishedYes
Event7th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2008 - Beijing, China
Duration: Apr 22 2008Apr 25 2008

Other

Other7th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2008
CountryChina
CityBeijing
Period4/22/084/25/08

Fingerprint

Metabolites
Tendons
Biomarkers
Gas chromatography
Liver
Pattern recognition
Mass spectrometry
Ions

Keywords

  • gas chromatography and mass spectrometry (GC-MS)
  • metabonomics
  • Osteoarthritis(OA)
  • pattern recognition
  • study of the nature of TCM syndromes
  • urinary metabolite profile

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Study of the urinary metabolite profile associated with osteoarthritis and the correlation with its TCM syndromes. / Chu, Li Xi; Yang, Song Bin; Jia, Wei; Qiu, Yunping; Su, Ming Ming.

IFMBE Proceedings. Vol. 19 IFMBE 2008. p. 691-696.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chu, LX, Yang, SB, Jia, W, Qiu, Y & Su, MM 2008, Study of the urinary metabolite profile associated with osteoarthritis and the correlation with its TCM syndromes. in IFMBE Proceedings. vol. 19 IFMBE, pp. 691-696, 7th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2008, Beijing, China, 4/22/08. https://doi.org/10.1007/978-3-540-79039-6-171
Chu, Li Xi ; Yang, Song Bin ; Jia, Wei ; Qiu, Yunping ; Su, Ming Ming. / Study of the urinary metabolite profile associated with osteoarthritis and the correlation with its TCM syndromes. IFMBE Proceedings. Vol. 19 IFMBE 2008. pp. 691-696
@inproceedings{21439273d6f14525b6245edcbae25fc9,
title = "Study of the urinary metabolite profile associated with osteoarthritis and the correlation with its TCM syndromes",
abstract = "To compare the distinction of endogenous small molecular metabolites in urine samples from the patients suffering from osteoarthritis(OA) and the healthy people. To contrast the variance of metabolic phenotypes belong to the two different TCM syndromes of OA. Methods: Urine samples of 85 participants (43 non-OA controls and 42 individuals with the disturbance of OA which include two different classes in TCM theory) were selected. Utilizing the combined gas chromatography and mass spectrometry (GC-MS) method for the detection of such urine samples. Pattern recognition was applied to analyze the raw data. Some potential biomarkers of OA were identified by means of retrieval of mass spectral database. By the same means, comparative study was carried out between the two groups of TCM syndromes. Results: Obvious differences were found in the total ion chromatograms (TIC) of urine samples between the OA and control subjects. When compared the insufficiency of liver and kidney with tendon and vessel stagnation syndrome group with the control, the plot of PCA scores showed that satisfactory separation was made. Better division was realized when the more sophisticated model PLSDA and OPLSDA were utilized. Well-pleasing separation was achieved when the comparison between of the asthenia of both spleen and kidney with dampness invading joints syndrome group and the control one as well as between of the two TCM syndromes. Conclusion: There are differences in the urine metabolite profiles among different types of syndrome of OA and the control group. The metabolic regulatory network in organism probably is perturbed in the progress of OA. The nature of types of syndrome of TCM maybe base on the variation of metabolites in vivo and the technique of metabonomics could facilitate the research of the nature of TCM theory.",
keywords = "gas chromatography and mass spectrometry (GC-MS), metabonomics, Osteoarthritis(OA), pattern recognition, study of the nature of TCM syndromes, urinary metabolite profile",
author = "Chu, {Li Xi} and Yang, {Song Bin} and Wei Jia and Yunping Qiu and Su, {Ming Ming}",
year = "2008",
doi = "10.1007/978-3-540-79039-6-171",
language = "English (US)",
isbn = "9783540790389",
volume = "19 IFMBE",
pages = "691--696",
booktitle = "IFMBE Proceedings",

}

TY - GEN

T1 - Study of the urinary metabolite profile associated with osteoarthritis and the correlation with its TCM syndromes

AU - Chu, Li Xi

AU - Yang, Song Bin

AU - Jia, Wei

AU - Qiu, Yunping

AU - Su, Ming Ming

PY - 2008

Y1 - 2008

N2 - To compare the distinction of endogenous small molecular metabolites in urine samples from the patients suffering from osteoarthritis(OA) and the healthy people. To contrast the variance of metabolic phenotypes belong to the two different TCM syndromes of OA. Methods: Urine samples of 85 participants (43 non-OA controls and 42 individuals with the disturbance of OA which include two different classes in TCM theory) were selected. Utilizing the combined gas chromatography and mass spectrometry (GC-MS) method for the detection of such urine samples. Pattern recognition was applied to analyze the raw data. Some potential biomarkers of OA were identified by means of retrieval of mass spectral database. By the same means, comparative study was carried out between the two groups of TCM syndromes. Results: Obvious differences were found in the total ion chromatograms (TIC) of urine samples between the OA and control subjects. When compared the insufficiency of liver and kidney with tendon and vessel stagnation syndrome group with the control, the plot of PCA scores showed that satisfactory separation was made. Better division was realized when the more sophisticated model PLSDA and OPLSDA were utilized. Well-pleasing separation was achieved when the comparison between of the asthenia of both spleen and kidney with dampness invading joints syndrome group and the control one as well as between of the two TCM syndromes. Conclusion: There are differences in the urine metabolite profiles among different types of syndrome of OA and the control group. The metabolic regulatory network in organism probably is perturbed in the progress of OA. The nature of types of syndrome of TCM maybe base on the variation of metabolites in vivo and the technique of metabonomics could facilitate the research of the nature of TCM theory.

AB - To compare the distinction of endogenous small molecular metabolites in urine samples from the patients suffering from osteoarthritis(OA) and the healthy people. To contrast the variance of metabolic phenotypes belong to the two different TCM syndromes of OA. Methods: Urine samples of 85 participants (43 non-OA controls and 42 individuals with the disturbance of OA which include two different classes in TCM theory) were selected. Utilizing the combined gas chromatography and mass spectrometry (GC-MS) method for the detection of such urine samples. Pattern recognition was applied to analyze the raw data. Some potential biomarkers of OA were identified by means of retrieval of mass spectral database. By the same means, comparative study was carried out between the two groups of TCM syndromes. Results: Obvious differences were found in the total ion chromatograms (TIC) of urine samples between the OA and control subjects. When compared the insufficiency of liver and kidney with tendon and vessel stagnation syndrome group with the control, the plot of PCA scores showed that satisfactory separation was made. Better division was realized when the more sophisticated model PLSDA and OPLSDA were utilized. Well-pleasing separation was achieved when the comparison between of the asthenia of both spleen and kidney with dampness invading joints syndrome group and the control one as well as between of the two TCM syndromes. Conclusion: There are differences in the urine metabolite profiles among different types of syndrome of OA and the control group. The metabolic regulatory network in organism probably is perturbed in the progress of OA. The nature of types of syndrome of TCM maybe base on the variation of metabolites in vivo and the technique of metabonomics could facilitate the research of the nature of TCM theory.

KW - gas chromatography and mass spectrometry (GC-MS)

KW - metabonomics

KW - Osteoarthritis(OA)

KW - pattern recognition

KW - study of the nature of TCM syndromes

KW - urinary metabolite profile

UR - http://www.scopus.com/inward/record.url?scp=78049370997&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049370997&partnerID=8YFLogxK

U2 - 10.1007/978-3-540-79039-6-171

DO - 10.1007/978-3-540-79039-6-171

M3 - Conference contribution

AN - SCOPUS:78049370997

SN - 9783540790389

VL - 19 IFMBE

SP - 691

EP - 696

BT - IFMBE Proceedings

ER -