Study of cells attachment using impedance spectroscopy technique

Anis N. Nordin, S. M. Arifuzzaman, Maizirwan Mel, David Spray, Ioana Voiculescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell-substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics™, a commercially available modeling package.

Original languageEnglish (US)
Title of host publicationBiomedical and Biotechnology Engineering; Nanoengineering for Medicine and Biology
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages931-935
Number of pages5
ISBN (Print)9780791854884
DOIs
StatePublished - 2011
EventASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011 - Denver, CO, United States
Duration: Nov 11 2011Nov 17 2011

Publication series

NameASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011
Volume2

Other

OtherASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011
Country/TerritoryUnited States
CityDenver, CO
Period11/11/1111/17/11

Keywords

  • COMSOL Multiphysics™
  • Cell-substrate impedance sensing (ECIS)
  • Eight-well cell culture impedance array
  • Impedance
  • Reactance

ASJC Scopus subject areas

  • Mechanical Engineering

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