Study of cells attachment using impedance spectroscopy technique

Anis N. Nordin, S. M. Arifuzzaman, Maizirwan Mel, David Spray, Ioana Voiculescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell-substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics™, a commercially available modeling package.

Original languageEnglish (US)
Title of host publicationASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011
Pages931-935
Number of pages5
StatePublished - Dec 1 2011
EventASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011 - Denver, CO, United States
Duration: Nov 11 2011Nov 17 2011

Publication series

NameASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011
Volume2

Other

OtherASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011
CountryUnited States
CityDenver, CO
Period11/11/1111/17/11

Keywords

  • COMSOL Multiphysics™
  • Cell-substrate impedance sensing (ECIS)
  • Eight-well cell culture impedance array
  • Impedance
  • Reactance

ASJC Scopus subject areas

  • Mechanical Engineering

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  • Cite this

    Nordin, A. N., Arifuzzaman, S. M., Mel, M., Spray, D., & Voiculescu, I. (2011). Study of cells attachment using impedance spectroscopy technique. In ASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011 (pp. 931-935). (ASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011; Vol. 2).