Study of antigen-processing steps reveals preferences explaining differential biological outcomes of two HLA-A2-restricted immunodominant epitopes from human immunodeficiency virus type 1

W. M. Cohen, A. Bianco, F. Connan, L. Camoin, M. Dalod, G. Lauvau, E. Ferriès, B. Culmann-Penciolelli, P. M. Van Endert, J. P. Briand, J. Choppin, J. G. Guillet

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Cytotoxic T-lymphocyte (CTL) responses directed to different human immunodeficiency virus (HIV) epitopes vary in their protective efficacy. In particular, HIV-infected cells are much more sensitive to lysis by anti-Gag/p17(77-85)/HLA-A2 than to that by anti-polymerase/RT(476-484)/HLA-A2 CTL, because of a higher density of p17(77-85) complexes. This report describes multiple processing steps favoring the generation of p17(77-85) complexes: (i) the exact COOH-terminal cleavage of epitopes by cellular proteases occurred faster and more frequently for p17(77-85) than for RT(476-484), and (ii) the binding efficiency of the transporter associated with antigen processing was greater for p17(77-85) precursors than for the RT(476-484) epitope. Surprisingly, these peptides, which differed markedly in their antigenicity, displayed qualitatively and quantitatively similar immunogenicity, suggesting differences in the mechanisms governing these phenomena. Here, we discuss the mechanisms responsible for such differences.

Original languageEnglish (US)
Pages (from-to)10219-10225
Number of pages7
JournalJournal of virology
Volume76
Issue number20
DOIs
StatePublished - Oct 2002

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Cohen, W. M., Bianco, A., Connan, F., Camoin, L., Dalod, M., Lauvau, G., Ferriès, E., Culmann-Penciolelli, B., Van Endert, P. M., Briand, J. P., Choppin, J., & Guillet, J. G. (2002). Study of antigen-processing steps reveals preferences explaining differential biological outcomes of two HLA-A2-restricted immunodominant epitopes from human immunodeficiency virus type 1. Journal of virology, 76(20), 10219-10225. https://doi.org/10.1128/JVI.76.20.10219-10225.2002