TY - JOUR
T1 - Studies of in vitro infection by Trypanosoma cruzi. I. Ultrastructural studies on the invasion of macrophages and L cells
AU - Tanowitz, H.
AU - Wittner, M.
AU - Kress, Y.
AU - Bloom, B.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1975
Y1 - 1975
N2 - The interactions of Trypanosoma cruzi with L cells, mouse subcutaneous fibroblasts, and with normal and activated macrophages in vitro were studied by ultrastructural techniques. T. cruzi actively invades cultured L cells and uniformly destroys them. Normal macrophages could control a 1:1 (parasite to host cell) infection, but were destroyed by a 10:1 infection. BCG activated macrophages, however, controlled a 10:1 infection but not one at a ratio of 100:1. It appears that parasites that survive within host cells do so outside cytoplasmic vacuoles, whereas when they are relegated to host cell phagosomes they are destroyed. Culture forms of T. cruzi have several means of access into host cells. Macrophages are better able to survive infection than are non phagocytic cells. Finally, it is suggested that control of an experimental infection in vitro is dependent upon numbers of parasites to macrophages as well as the state of the macrophages.
AB - The interactions of Trypanosoma cruzi with L cells, mouse subcutaneous fibroblasts, and with normal and activated macrophages in vitro were studied by ultrastructural techniques. T. cruzi actively invades cultured L cells and uniformly destroys them. Normal macrophages could control a 1:1 (parasite to host cell) infection, but were destroyed by a 10:1 infection. BCG activated macrophages, however, controlled a 10:1 infection but not one at a ratio of 100:1. It appears that parasites that survive within host cells do so outside cytoplasmic vacuoles, whereas when they are relegated to host cell phagosomes they are destroyed. Culture forms of T. cruzi have several means of access into host cells. Macrophages are better able to survive infection than are non phagocytic cells. Finally, it is suggested that control of an experimental infection in vitro is dependent upon numbers of parasites to macrophages as well as the state of the macrophages.
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U2 - 10.4269/ajtmh.1975.24.25
DO - 10.4269/ajtmh.1975.24.25
M3 - Article
C2 - 1089367
AN - SCOPUS:0016433975
SN - 0002-9637
VL - 24
SP - 25
EP - 33
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 1
ER -