Studies of in vitro infection by Trypanosoma cruzi. I. Ultrastructural studies on the invasion of macrophages and L cells

H. Tanowitz, M. Wittner, Y. Kress, B. Bloom

Research output: Contribution to journalArticle

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Abstract

The interactions of Trypanosoma cruzi with L cells, mouse subcutaneous fibroblasts, and with normal and activated macrophages in vitro were studied by ultrastructural techniques. T. cruzi actively invades cultured L cells and uniformly destroys them. Normal macrophages could control a 1:1 (parasite to host cell) infection, but were destroyed by a 10:1 infection. BCG activated macrophages, however, controlled a 10:1 infection but not one at a ratio of 100:1. It appears that parasites that survive within host cells do so outside cytoplasmic vacuoles, whereas when they are relegated to host cell phagosomes they are destroyed. Culture forms of T. cruzi have several means of access into host cells. Macrophages are better able to survive infection than are non phagocytic cells. Finally, it is suggested that control of an experimental infection in vitro is dependent upon numbers of parasites to macrophages as well as the state of the macrophages.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume24
Issue number1
StatePublished - 1975

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Trypanosoma cruzi
Macrophages
Infection
Parasites
Phagosomes
Phagocytes
Infection Control
Mycobacterium bovis
Vacuoles
In Vitro Techniques
Cultured Cells
Fibroblasts

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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Studies of in vitro infection by Trypanosoma cruzi. I. Ultrastructural studies on the invasion of macrophages and L cells. / Tanowitz, H.; Wittner, M.; Kress, Y.; Bloom, B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 24, No. 1, 1975, p. 25-33.

Research output: Contribution to journalArticle

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