Structures of the Michaelis complex (1.2 Å) and the covalent acyl intermediate (2.0 Å) of cefamandole bound in the active sites of the mycobacterium tuberculosis β-lactamase K73A and E166A mutants

Lee W. Tremblay, Hua Xu, John S. Blanchard

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The genome of Mycobacterium tuberculosis (TB) contains a gene that encodes a highly active β-lactamase, BlaC, that imparts TB with resistance to β-lactam chemotherapy. The structure of covalent BlaC-β-lactam complexes suggests that active site residues K73 and E166 are essential for acylation and deacylation, respectively. We have prepared the K73A and E166A mutant forms of BlaC and have determined the structures of the Michaelis complex of cefamandole and the covalently bound acyl intermediate of cefamandole at resolutions of 1.2 and 2.0 Å, respectively. These structures provide insight into the details of the catalytic mechanism.

Original languageEnglish (US)
Pages (from-to)9685-9687
Number of pages3
JournalBiochemistry
Volume49
Issue number45
DOIs
StatePublished - Nov 16 2010

ASJC Scopus subject areas

  • Biochemistry

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