Structure of the sodium channel pore revealed by serial cysteine mutagenesis

M. T. Pérez-García; N. Chiamvimonvat; E. Marban; G. F. Tomaselli

doi:10.1073/pnas.93.1.300

Structure of the sodium channel pore revealed by serial cysteine mutagenesis

M. T. Pérez-García, N. Chiamvimonvat, E. Marban, G. F. Tomaselli

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

The pores of voltage-gated cation channels are formed by four intramembrane segments that impart selectivity and conductance. Remarkably little is known about the higher order structure of these critical pure- lining or P segments. Serial cysteine mutagenesis reveals a pattern of side- chain accessibility that contradicts currently favored structural models based on α-helices or β-strands. Like the active sites of many enzymes of known structure, the sodium channel pore consists of irregular loop regions.

Original language	English (US)
Pages (from-to)	300-304
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	93
Issue number	1
DOIs	https://doi.org/10.1073/pnas.93.1.300
State	Published - Jan 9 1996
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.93.1.300

Cite this

@article{411fcba2f811498cb13f544b7cc61cc3,

title = "Structure of the sodium channel pore revealed by serial cysteine mutagenesis",

abstract = "The pores of voltage-gated cation channels are formed by four intramembrane segments that impart selectivity and conductance. Remarkably little is known about the higher order structure of these critical pure- lining or P segments. Serial cysteine mutagenesis reveals a pattern of side- chain accessibility that contradicts currently favored structural models based on α-helices or β-strands. Like the active sites of many enzymes of known structure, the sodium channel pore consists of irregular loop regions.",

author = "P{\'e}rez-Garc{\'i}a, {M. T.} and N. Chiamvimonvat and E. Marban and Tomaselli, {G. F.}",

year = "1996",

month = jan,

day = "9",

doi = "10.1073/pnas.93.1.300",

language = "English (US)",

volume = "93",

pages = "300--304",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "1",

}

TY - JOUR

T1 - Structure of the sodium channel pore revealed by serial cysteine mutagenesis

AU - Pérez-García, M. T.

AU - Chiamvimonvat, N.

AU - Marban, E.

AU - Tomaselli, G. F.

PY - 1996/1/9

Y1 - 1996/1/9

N2 - The pores of voltage-gated cation channels are formed by four intramembrane segments that impart selectivity and conductance. Remarkably little is known about the higher order structure of these critical pure- lining or P segments. Serial cysteine mutagenesis reveals a pattern of side- chain accessibility that contradicts currently favored structural models based on α-helices or β-strands. Like the active sites of many enzymes of known structure, the sodium channel pore consists of irregular loop regions.

AB - The pores of voltage-gated cation channels are formed by four intramembrane segments that impart selectivity and conductance. Remarkably little is known about the higher order structure of these critical pure- lining or P segments. Serial cysteine mutagenesis reveals a pattern of side- chain accessibility that contradicts currently favored structural models based on α-helices or β-strands. Like the active sites of many enzymes of known structure, the sodium channel pore consists of irregular loop regions.

UR - http://www.scopus.com/inward/record.url?scp=0030051135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030051135&partnerID=8YFLogxK

U2 - 10.1073/pnas.93.1.300

DO - 10.1073/pnas.93.1.300

M3 - Article

C2 - 8552626

AN - SCOPUS:0030051135

SN - 0027-8424

VL - 93

SP - 300

EP - 304

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 1

ER -

Structure of the sodium channel pore revealed by serial cysteine mutagenesis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this