Structure of the Rhodobacter sphaeroides light-harvesting 1 β subunit in detergent micelles

P. L. Sorgen, Sean M. Cahill, R. D. Krueger-Koplin, S. T. Krueger-Koplin, C. C. Schenck, M. E. Girvin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The light harvesting 1 antenna (LH1) complex from Rhodobacter sphaeroides funnels excitation energy to the photosynthetic reaction center. Our ultimate goal is to build up the structure of LH1 from structures of its individual subunits, much as the antenna can self-assemble from its components in membrane-mimicking detergent micelles. The β subunit adopts a nativelike conformation in Zwittergent 3:12 micelles as demonstrated by its ability to take the first step of assembly, binding BChl a. Multidimensional NMR spectroscopy shows that the β subunit folds as a helix(L12-S25)-hinge(G26-W28)helix(L29-W44) structure with the helical regions for the 10 lowest-energy structures having backbone rmsds of 0.26 and 0.24 Å, respectively. Mn2+ relaxation data and the protein - detergent NOE pattern show the C-terminal helix embedded in the micelle and the N-terminal helix lying along the detergent micelle surface with a 60° angle between their long axes. 15N relaxation data for residues L12-W44 are typical of a well-ordered protein with a correlation time of 8.25 ± 2.1 ns. The presence of the hinge region placing the N-terminal helix along the membrane surface may be the structural feature responsible for the functional differences observed between the LH1 and LH2 β subunits.

Original languageEnglish (US)
Pages (from-to)31-41
Number of pages11
JournalBiochemistry
Volume41
Issue number1
DOIs
StatePublished - Jan 8 2002

Fingerprint

Rhodobacter sphaeroides
Micelles
Detergents
L 012
Antennas
Light
Hinges
Light-Harvesting Protein Complexes
Photosynthetic Reaction Center Complex Proteins
Membranes
Excitation energy
Nuclear magnetic resonance spectroscopy
Conformations
Proteins
Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sorgen, P. L., Cahill, S. M., Krueger-Koplin, R. D., Krueger-Koplin, S. T., Schenck, C. C., & Girvin, M. E. (2002). Structure of the Rhodobacter sphaeroides light-harvesting 1 β subunit in detergent micelles. Biochemistry, 41(1), 31-41. https://doi.org/10.1021/bi011576j

Structure of the Rhodobacter sphaeroides light-harvesting 1 β subunit in detergent micelles. / Sorgen, P. L.; Cahill, Sean M.; Krueger-Koplin, R. D.; Krueger-Koplin, S. T.; Schenck, C. C.; Girvin, M. E.

In: Biochemistry, Vol. 41, No. 1, 08.01.2002, p. 31-41.

Research output: Contribution to journalArticle

Sorgen, PL, Cahill, SM, Krueger-Koplin, RD, Krueger-Koplin, ST, Schenck, CC & Girvin, ME 2002, 'Structure of the Rhodobacter sphaeroides light-harvesting 1 β subunit in detergent micelles', Biochemistry, vol. 41, no. 1, pp. 31-41. https://doi.org/10.1021/bi011576j
Sorgen PL, Cahill SM, Krueger-Koplin RD, Krueger-Koplin ST, Schenck CC, Girvin ME. Structure of the Rhodobacter sphaeroides light-harvesting 1 β subunit in detergent micelles. Biochemistry. 2002 Jan 8;41(1):31-41. https://doi.org/10.1021/bi011576j
Sorgen, P. L. ; Cahill, Sean M. ; Krueger-Koplin, R. D. ; Krueger-Koplin, S. T. ; Schenck, C. C. ; Girvin, M. E. / Structure of the Rhodobacter sphaeroides light-harvesting 1 β subunit in detergent micelles. In: Biochemistry. 2002 ; Vol. 41, No. 1. pp. 31-41.
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