Structure of the actin crosslinking core of fimbrin

Michael G. Klein, Wuxian Shi, Udupi Ramagopal, Yiider Tseng, Denis Wirtz, David R. Kovar, Christopher J. Staiger, Steven C. Almo

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Filamentous actin is organized into bundles and orthogonal networks by the fimbrin/α-actinin superfamily of F-actin crosslinking proteins. The crystal structure of the Arabidopsis thaliana and Schizosaccharomyces pombe fimbrin cores provides the first description of a functional F-actin crosslinking protein and highlights the compact and distinctly asymmetric organization of the fimbrin molecule, in which the two actin binding domains present distinct surfaces to solvent. The mapping of functionally important residues onto the structure affords new insights into the binding process and provides additional constraints which must be accommodated by models for F-actin binding and crosslinking. Most strikingly, this work provides unique insight into the mechanistic features of conditional-lethal mutants and their extragenic suppressors, which highlight conformational and dynamic properties required for fimbrin function. These results underscore the power of jointly considering structural and genetic suppressor data for obtaining unexpected and biologically relevant mechanistic information.

Original languageEnglish (US)
Pages (from-to)999-1013
Number of pages15
JournalStructure
Volume12
Issue number6
DOIs
StatePublished - Jun 2004

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Actins
Actinin
Schizosaccharomyces
Arabidopsis
Proteins
plastin

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Klein, M. G., Shi, W., Ramagopal, U., Tseng, Y., Wirtz, D., Kovar, D. R., ... Almo, S. C. (2004). Structure of the actin crosslinking core of fimbrin. Structure, 12(6), 999-1013. https://doi.org/10.1016/j.str.2004.04.010

Structure of the actin crosslinking core of fimbrin. / Klein, Michael G.; Shi, Wuxian; Ramagopal, Udupi; Tseng, Yiider; Wirtz, Denis; Kovar, David R.; Staiger, Christopher J.; Almo, Steven C.

In: Structure, Vol. 12, No. 6, 06.2004, p. 999-1013.

Research output: Contribution to journalArticle

Klein, MG, Shi, W, Ramagopal, U, Tseng, Y, Wirtz, D, Kovar, DR, Staiger, CJ & Almo, SC 2004, 'Structure of the actin crosslinking core of fimbrin', Structure, vol. 12, no. 6, pp. 999-1013. https://doi.org/10.1016/j.str.2004.04.010
Klein MG, Shi W, Ramagopal U, Tseng Y, Wirtz D, Kovar DR et al. Structure of the actin crosslinking core of fimbrin. Structure. 2004 Jun;12(6):999-1013. https://doi.org/10.1016/j.str.2004.04.010
Klein, Michael G. ; Shi, Wuxian ; Ramagopal, Udupi ; Tseng, Yiider ; Wirtz, Denis ; Kovar, David R. ; Staiger, Christopher J. ; Almo, Steven C. / Structure of the actin crosslinking core of fimbrin. In: Structure. 2004 ; Vol. 12, No. 6. pp. 999-1013.
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