Structure of nectin-2 reveals determinants of homophilic and heterophilic interactions that control cell-cell adhesion

Dibyendu Samanta, Udupi A. Ramagopal, Rotem Rubinstein, Vladimir Vigdorovich, Stanley G. Nathenson, Steven C. Almo

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Nectins are members of the Ig superfamily that mediate cell-cell adhesion through homophilic and heterophilic interactions. We have determined the crystal structure of the nectin-2 homodimer at 1.3 Å resolution. Structural analysis and complementary mutagenesis studies reveal the basis for recognition and selectivity among the nectin family members. Notably, the close proximity of charged residues at the dimer interface is a major determinant of the binding affinities associated with homophilic and heterophilic interactions within the nectin family. Our structural and biochemical data provide a mechanistic basis to explain stronger heterophilic versus weaker homophilic interactions among these family members and also offer insights into nectin-mediated transinteractions between engaging cells.

Original languageEnglish (US)
Pages (from-to)14836-14840
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number37
DOIs
StatePublished - Sep 11 2012

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Cell Adhesion
Cell Communication
Mutagenesis
nectins

Keywords

  • Cell adhesion molecule
  • Immunoglobulin superfamily
  • Nectin-2 dimer
  • X-ray crystallography

ASJC Scopus subject areas

  • General

Cite this

Structure of nectin-2 reveals determinants of homophilic and heterophilic interactions that control cell-cell adhesion. / Samanta, Dibyendu; Ramagopal, Udupi A.; Rubinstein, Rotem; Vigdorovich, Vladimir; Nathenson, Stanley G.; Almo, Steven C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 37, 11.09.2012, p. 14836-14840.

Research output: Contribution to journalArticle

Samanta, Dibyendu ; Ramagopal, Udupi A. ; Rubinstein, Rotem ; Vigdorovich, Vladimir ; Nathenson, Stanley G. ; Almo, Steven C. / Structure of nectin-2 reveals determinants of homophilic and heterophilic interactions that control cell-cell adhesion. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 37. pp. 14836-14840.
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