Structure of mutant human carbonmonoxyhemoglobin C (βE6K) at 2.0 Å resolution

John C. Dewan, Angela Feeling-Taylor, Yoram A. Puius, Larysa Patskovska, Yury Patskovsky, Ronald L. Nagel, Steven C. Almo, Rhoda Elison Hirsch

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The first high-resolution crystal structure for liganded hemoglobin C, a mutant hemoglobin that forms crystals in red blood cells of individuals with CC and SC diseases, was obtained from a COHbC crystal grown in concentrated potassium phosphate buffer. The dynamic structure of the βEK6 side chain could potentially support a number of specific polar interactions that may contribute to enhanced nucleation kinetics for HbC relative to HbA.

Original languageEnglish (US)
Pages (from-to)2038-2042
Number of pages5
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number12
DOIs
StatePublished - Dec 1 2002

Fingerprint

Hemoglobin SC Disease
Hemoglobin C
Carboxyhemoglobin
hemoglobin
Buffers
Hemoglobins
Erythrocytes
potassium phosphates
Crystals
erythrocytes
crystals
Blood
Nucleation
buffers
Crystal structure
Cells
nucleation
crystal structure
Kinetics
high resolution

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Structure of mutant human carbonmonoxyhemoglobin C (βE6K) at 2.0 Å resolution. / Dewan, John C.; Feeling-Taylor, Angela; Puius, Yoram A.; Patskovska, Larysa; Patskovsky, Yury; Nagel, Ronald L.; Almo, Steven C.; Hirsch, Rhoda Elison.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 58, No. 12, 01.12.2002, p. 2038-2042.

Research output: Contribution to journalArticle

Dewan, John C. ; Feeling-Taylor, Angela ; Puius, Yoram A. ; Patskovska, Larysa ; Patskovsky, Yury ; Nagel, Ronald L. ; Almo, Steven C. ; Hirsch, Rhoda Elison. / Structure of mutant human carbonmonoxyhemoglobin C (βE6K) at 2.0 Å resolution. In: Acta Crystallographica Section D: Biological Crystallography. 2002 ; Vol. 58, No. 12. pp. 2038-2042.
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