Structure of hamster insulin: comparison with a tumor insulin

F. A. Neelon, H. K. Delcher, Howard M. Steinman, H. E. Lebovitz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Insulin, isolated from hamster pancreata by acid ethanol extraction, membrane ultrafiltration, DEAE cellulose and Sephadex G50 chromatography, was pure and used for sequence studies. Amino acid sequence was established by tryptic cleavage and both manual and automatic Edman degradation. The proposed B chain sequence is identical with that of rabbit insulin, while A chain is identical to that of rat. However, this specific combination of A and B chains is unique. An insulin immunoreactive peptide was isolated from a transplantable hamster pancreatic carcinoma by a similar process. Chromatographic behavior was identical to that of normal hamster insulin but the tumor preparation was contaminated with peptides which were only removed after sulfitolysis of the tumor insulin to respective A and B chains. Amino acid composition and partial sequence data show that the tumor product is identical to the normal pancreatic insulin.

Original languageEnglish (US)
JournalFederation Proceedings
Volume32
Issue number3
StatePublished - 1973
Externally publishedYes

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Cricetinae
Insulin
Neoplasms
DEAE-Dextran
DEAE-Cellulose
Peptides
Ultrafiltration
Chromatography
Pancreas
Amino Acid Sequence
Ethanol
Rabbits
Amino Acids
Acids
Membranes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Neelon, F. A., Delcher, H. K., Steinman, H. M., & Lebovitz, H. E. (1973). Structure of hamster insulin: comparison with a tumor insulin. Federation Proceedings, 32(3).

Structure of hamster insulin : comparison with a tumor insulin. / Neelon, F. A.; Delcher, H. K.; Steinman, Howard M.; Lebovitz, H. E.

In: Federation Proceedings, Vol. 32, No. 3, 1973.

Research output: Contribution to journalArticle

Neelon, FA, Delcher, HK, Steinman, HM & Lebovitz, HE 1973, 'Structure of hamster insulin: comparison with a tumor insulin', Federation Proceedings, vol. 32, no. 3.
Neelon, F. A. ; Delcher, H. K. ; Steinman, Howard M. ; Lebovitz, H. E. / Structure of hamster insulin : comparison with a tumor insulin. In: Federation Proceedings. 1973 ; Vol. 32, No. 3.
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