Structure determination of turkey egg-white lysozyme using Laue diffraction data.

P. L. Howell, Steven C. Almo, M. R. Parsons, J. Hajdu, G. A. Petsko

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The three-dimensional structure of turkey egg-white lysozyme (TEWL) has been solved and refined at 2.5 A resolution using X-ray data collected by the Laue method. This is the first protein structure determination undertaken using Laue diffraction data. A re-examination of the existing structure of TEWL was necessary when attempts to refine an atomic model based on the C alpha positions in the Protein Data Bank (entry 1LZ2) failed. The correct orientation and position of the turkey lysozyme molecules within the crystallographic unit cell were determined by molecular replacement using a refined model of the homologous hen egg-white lysozyme crystal structure. After modification of the model to reflect the differences in amino-acid sequence between the chicken and turkey enzymes, the structure was subjected to crystallographic refinement using the simulated-annealing refinement technique and conventional least-squares refinement. This yielded a final residual of R = 20.7%. This crystal form is of potential interest for time-resolved crystallographic studies since the amino-acid residues involved in catalysis (Asp52 and Glu35) are accessible to solvent and not blocked by crystal contacts.

Original languageEnglish (US)
JournalActa crystallographica. Section B, Structural science
Volume48
DOIs
StatePublished - Apr 1 1992
Externally publishedYes

Fingerprint

Egg White
eggs
lysozyme
Muramidase
Diffraction
diffraction
amino acids
Laue method
proteins
Amino Acids
chickens
Crystals
simulated annealing
Simulated annealing
Least-Squares Analysis
Catalysis
entry
catalysis
crystals
enzymes

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Structural Biology

Cite this

Structure determination of turkey egg-white lysozyme using Laue diffraction data. / Howell, P. L.; Almo, Steven C.; Parsons, M. R.; Hajdu, J.; Petsko, G. A.

In: Acta crystallographica. Section B, Structural science, Vol. 48, 01.04.1992.

Research output: Contribution to journalArticle

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