Structure and replication of the genome of the hepatitis δ virus

P. J. Chen, Ganjam V. Kalpana, J. Goldberg, W. Mason, B. Werner, J. Gerin, J. Taylor

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

The hepatitis δ virus can be found in the serum and liver of some hepatitis B virus patients. We now report that the RNA genome of serum-derived δ virus is single-stranded and circular. Livers of infected chimpanzees or woodchucks contained as many as 300,000 copies of genomic strand RNA per average cell, and at least some of this RNA had a circular conformation. Also present in the liver were RNA species complementary to the virion RNA. The genomic RNA was 5-22 times more abundant than this antigenomic strand. Some of the antigenomic RNA was complexed with genomic RNA, as evidenced by the fact that at least 34% of the antigenomic RNA was resistant to digestion with either RNase A in 0.3 M NaCl or S1 nuclease. Some of the antigenomic RNA was in a circular conformation. These and other findings showed that the structure and replication of hepatitis δ virus are in many ways similar to those of the previously described plant viroids, virusoids, and satellite RNAs.

Original languageEnglish (US)
Pages (from-to)8774-8778
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number22
DOIs
StatePublished - 1986
Externally publishedYes

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Hepatitis Viruses
Genome
RNA
Liver
Satellite RNAs
Viroids
Marmota
Complementary RNA
Pancreatic Ribonuclease
Pan troglodytes
Serum
Hepatitis B virus
Virion
Digestion
Viruses

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Structure and replication of the genome of the hepatitis δ virus. / Chen, P. J.; Kalpana, Ganjam V.; Goldberg, J.; Mason, W.; Werner, B.; Gerin, J.; Taylor, J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 22, 1986, p. 8774-8778.

Research output: Contribution to journalArticle

Chen, P. J. ; Kalpana, Ganjam V. ; Goldberg, J. ; Mason, W. ; Werner, B. ; Gerin, J. ; Taylor, J. / Structure and replication of the genome of the hepatitis δ virus. In: Proceedings of the National Academy of Sciences of the United States of America. 1986 ; Vol. 83, No. 22. pp. 8774-8778.
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