TY - JOUR
T1 - Structure and replication of the genome of the hepatitis δ virus
AU - Chen, P. J.
AU - Kalpana, G.
AU - Goldberg, J.
AU - Mason, W.
AU - Werner, B.
AU - Gerin, J.
AU - Taylor, J.
PY - 1986
Y1 - 1986
N2 - The hepatitis δ virus can be found in the serum and liver of some hepatitis B virus patients. We now report that the RNA genome of serum-derived δ virus is single-stranded and circular. Livers of infected chimpanzees or woodchucks contained as many as 300,000 copies of genomic strand RNA per average cell, and at least some of this RNA had a circular conformation. Also present in the liver were RNA species complementary to the virion RNA. The genomic RNA was 5-22 times more abundant than this antigenomic strand. Some of the antigenomic RNA was complexed with genomic RNA, as evidenced by the fact that at least 34% of the antigenomic RNA was resistant to digestion with either RNase A in 0.3 M NaCl or S1 nuclease. Some of the antigenomic RNA was in a circular conformation. These and other findings showed that the structure and replication of hepatitis δ virus are in many ways similar to those of the previously described plant viroids, virusoids, and satellite RNAs.
AB - The hepatitis δ virus can be found in the serum and liver of some hepatitis B virus patients. We now report that the RNA genome of serum-derived δ virus is single-stranded and circular. Livers of infected chimpanzees or woodchucks contained as many as 300,000 copies of genomic strand RNA per average cell, and at least some of this RNA had a circular conformation. Also present in the liver were RNA species complementary to the virion RNA. The genomic RNA was 5-22 times more abundant than this antigenomic strand. Some of the antigenomic RNA was complexed with genomic RNA, as evidenced by the fact that at least 34% of the antigenomic RNA was resistant to digestion with either RNase A in 0.3 M NaCl or S1 nuclease. Some of the antigenomic RNA was in a circular conformation. These and other findings showed that the structure and replication of hepatitis δ virus are in many ways similar to those of the previously described plant viroids, virusoids, and satellite RNAs.
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U2 - 10.1073/pnas.83.22.8774
DO - 10.1073/pnas.83.22.8774
M3 - Article
C2 - 2430299
AN - SCOPUS:1542774842
SN - 0027-8424
VL - 83
SP - 8774
EP - 8778
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22
ER -
