Abstract
Fluorescence polarization microscopy (FPM) is the analysis of the polarization of light in a fluorescent microscope in order to determine the angular orientation and rotational mobility of fluorescent molecules. Key advantages of FPM, relative to other structural analysis techniques, are that it allows the detection of conformational changes of fluorescently labeled macromolecules in real time in physiological conditions and at the single-molecule level. In this chapter we describe in detail the FPM experimental set-up and analysis methods we have used to investigate structural intermediates of the motor protein kinesin-1 associated with its walking mechanism along microtubules. We also briefly describe additional FPM methods that have been used to investigate other macromolecular complexes.
| Original language | English (US) |
|---|---|
| Publisher | Unknown Publisher |
| Number of pages | 15 |
| Volume | 95 |
| Edition | C |
| DOIs | |
| State | Published - 2010 |
Publication series
| Name | Methods in Cell Biology |
|---|---|
| No. | C |
| Volume | 95 |
| ISSN (Print) | 0091679X |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy. / Sosa, Hernando J.; Asenjo, Ana B.; Peterman, Erwin J G.
C ed. Unknown Publisher, 2010. 15 p. (Methods in Cell Biology; Vol. 95, No. C).Research output: Book/Report › Book
}
TY - BOOK
T1 - Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy
AU - Sosa, Hernando J.
AU - Asenjo, Ana B.
AU - Peterman, Erwin J G
PY - 2010
Y1 - 2010
N2 - Fluorescence polarization microscopy (FPM) is the analysis of the polarization of light in a fluorescent microscope in order to determine the angular orientation and rotational mobility of fluorescent molecules. Key advantages of FPM, relative to other structural analysis techniques, are that it allows the detection of conformational changes of fluorescently labeled macromolecules in real time in physiological conditions and at the single-molecule level. In this chapter we describe in detail the FPM experimental set-up and analysis methods we have used to investigate structural intermediates of the motor protein kinesin-1 associated with its walking mechanism along microtubules. We also briefly describe additional FPM methods that have been used to investigate other macromolecular complexes.
AB - Fluorescence polarization microscopy (FPM) is the analysis of the polarization of light in a fluorescent microscope in order to determine the angular orientation and rotational mobility of fluorescent molecules. Key advantages of FPM, relative to other structural analysis techniques, are that it allows the detection of conformational changes of fluorescently labeled macromolecules in real time in physiological conditions and at the single-molecule level. In this chapter we describe in detail the FPM experimental set-up and analysis methods we have used to investigate structural intermediates of the motor protein kinesin-1 associated with its walking mechanism along microtubules. We also briefly describe additional FPM methods that have been used to investigate other macromolecular complexes.
UR - http://www.scopus.com/inward/record.url?scp=77955283864&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955283864&partnerID=8YFLogxK
U2 - 10.1016/S0091-679X(10)95025-5
DO - 10.1016/S0091-679X(10)95025-5
M3 - Book
VL - 95
T3 - Methods in Cell Biology
BT - Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy
PB - Unknown Publisher
ER -