Structural shifts of gut microbiota as surrogate endpoints for monitoring host health changes induced by carcinogen exposure

Hua Wei, Li Dong, Tingting Wang, Menghui Zhang, Weiying Hua, Chenhong Zhang, Xiaoyan Pang, Minjun Chen, Mingming Su, Yunping Qiu, Mingmei Zhou, Shengli Yang, Zhu Chen, Mattias Rantalainen, Jeremy K. Nicholson, Wei Jia, Dazheng Wu, Liping Zhao

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

This study monitored structural shifts of gut microbiota of rats developing precancerous mucosal lesions induced by carcinogen 1,2-dimethyl hydrazine (DMH) treatment using PCR-denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing on the 16S rRNA gene V3 region. Partial least square discriminant analysis of DGGE fingerprints showed that the gut microbiota structure of treated animals was similar to that of the controls 1 and 3 weeks after DMH treatments, but significantly different 7 weeks after DMH treatments, when a large number of aberrant crypt foci (ACF) developed in their colons. Martens' uncertainty test, followed by anova test (P<0.05) identified Ruminococcus-like and Allobaculum-like bacteria as key variables for discrimination of DMH-treated rats from controls. Real-time PCR confirmed the significant increase of the Ruminococcus obeum and the Allobaculum-like bacteria in DMH-treated rats. UniFrac analysis based on V3 pyrosequencing further validated that the gut microbiota structures of treated and control animals were similar at an early stage, but segregated after ACF formation. Thirteen operational taxonomic units including Ruminococcus-like and Allobaculum-like bacteria were identified as key variables for the discrimination of DMH-treated rats from controls. Dynamic analysis of gut microbiota may become a noninvasive strategy for monitoring host health changes induced by carcinogen exposure.

Original languageEnglish (US)
Pages (from-to)577-586
Number of pages10
JournalFEMS Microbiology Ecology
Volume73
Issue number3
DOIs
StatePublished - Sep 2010
Externally publishedYes

Fingerprint

hydrazine
carcinogen
Carcinogens
Ruminococcus
Biomarkers
bacterium
Aberrant Crypt Foci
electrokinesis
Health
monitoring
gel
Denaturing Gradient Gel Electrophoresis
Bacteria
animal
dynamic analysis
discriminant analysis
lesion
1,2-Dimethylhydrazine
Mustelidae
Animal Structures

Keywords

  • colorectal cancer
  • gut microbiota
  • health assessment

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Medicine(all)

Cite this

Structural shifts of gut microbiota as surrogate endpoints for monitoring host health changes induced by carcinogen exposure. / Wei, Hua; Dong, Li; Wang, Tingting; Zhang, Menghui; Hua, Weiying; Zhang, Chenhong; Pang, Xiaoyan; Chen, Minjun; Su, Mingming; Qiu, Yunping; Zhou, Mingmei; Yang, Shengli; Chen, Zhu; Rantalainen, Mattias; Nicholson, Jeremy K.; Jia, Wei; Wu, Dazheng; Zhao, Liping.

In: FEMS Microbiology Ecology, Vol. 73, No. 3, 09.2010, p. 577-586.

Research output: Contribution to journalArticle

Wei, H, Dong, L, Wang, T, Zhang, M, Hua, W, Zhang, C, Pang, X, Chen, M, Su, M, Qiu, Y, Zhou, M, Yang, S, Chen, Z, Rantalainen, M, Nicholson, JK, Jia, W, Wu, D & Zhao, L 2010, 'Structural shifts of gut microbiota as surrogate endpoints for monitoring host health changes induced by carcinogen exposure', FEMS Microbiology Ecology, vol. 73, no. 3, pp. 577-586. https://doi.org/10.1111/j.1574-6941.2010.00924.x
Wei, Hua ; Dong, Li ; Wang, Tingting ; Zhang, Menghui ; Hua, Weiying ; Zhang, Chenhong ; Pang, Xiaoyan ; Chen, Minjun ; Su, Mingming ; Qiu, Yunping ; Zhou, Mingmei ; Yang, Shengli ; Chen, Zhu ; Rantalainen, Mattias ; Nicholson, Jeremy K. ; Jia, Wei ; Wu, Dazheng ; Zhao, Liping. / Structural shifts of gut microbiota as surrogate endpoints for monitoring host health changes induced by carcinogen exposure. In: FEMS Microbiology Ecology. 2010 ; Vol. 73, No. 3. pp. 577-586.
@article{b804d5377b4146cfbcd608f079bc5e8b,
title = "Structural shifts of gut microbiota as surrogate endpoints for monitoring host health changes induced by carcinogen exposure",
abstract = "This study monitored structural shifts of gut microbiota of rats developing precancerous mucosal lesions induced by carcinogen 1,2-dimethyl hydrazine (DMH) treatment using PCR-denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing on the 16S rRNA gene V3 region. Partial least square discriminant analysis of DGGE fingerprints showed that the gut microbiota structure of treated animals was similar to that of the controls 1 and 3 weeks after DMH treatments, but significantly different 7 weeks after DMH treatments, when a large number of aberrant crypt foci (ACF) developed in their colons. Martens' uncertainty test, followed by anova test (P<0.05) identified Ruminococcus-like and Allobaculum-like bacteria as key variables for discrimination of DMH-treated rats from controls. Real-time PCR confirmed the significant increase of the Ruminococcus obeum and the Allobaculum-like bacteria in DMH-treated rats. UniFrac analysis based on V3 pyrosequencing further validated that the gut microbiota structures of treated and control animals were similar at an early stage, but segregated after ACF formation. Thirteen operational taxonomic units including Ruminococcus-like and Allobaculum-like bacteria were identified as key variables for the discrimination of DMH-treated rats from controls. Dynamic analysis of gut microbiota may become a noninvasive strategy for monitoring host health changes induced by carcinogen exposure.",
keywords = "colorectal cancer, gut microbiota, health assessment",
author = "Hua Wei and Li Dong and Tingting Wang and Menghui Zhang and Weiying Hua and Chenhong Zhang and Xiaoyan Pang and Minjun Chen and Mingming Su and Yunping Qiu and Mingmei Zhou and Shengli Yang and Zhu Chen and Mattias Rantalainen and Nicholson, {Jeremy K.} and Wei Jia and Dazheng Wu and Liping Zhao",
year = "2010",
month = "9",
doi = "10.1111/j.1574-6941.2010.00924.x",
language = "English (US)",
volume = "73",
pages = "577--586",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Structural shifts of gut microbiota as surrogate endpoints for monitoring host health changes induced by carcinogen exposure

AU - Wei, Hua

AU - Dong, Li

AU - Wang, Tingting

AU - Zhang, Menghui

AU - Hua, Weiying

AU - Zhang, Chenhong

AU - Pang, Xiaoyan

AU - Chen, Minjun

AU - Su, Mingming

AU - Qiu, Yunping

AU - Zhou, Mingmei

AU - Yang, Shengli

AU - Chen, Zhu

AU - Rantalainen, Mattias

AU - Nicholson, Jeremy K.

AU - Jia, Wei

AU - Wu, Dazheng

AU - Zhao, Liping

PY - 2010/9

Y1 - 2010/9

N2 - This study monitored structural shifts of gut microbiota of rats developing precancerous mucosal lesions induced by carcinogen 1,2-dimethyl hydrazine (DMH) treatment using PCR-denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing on the 16S rRNA gene V3 region. Partial least square discriminant analysis of DGGE fingerprints showed that the gut microbiota structure of treated animals was similar to that of the controls 1 and 3 weeks after DMH treatments, but significantly different 7 weeks after DMH treatments, when a large number of aberrant crypt foci (ACF) developed in their colons. Martens' uncertainty test, followed by anova test (P<0.05) identified Ruminococcus-like and Allobaculum-like bacteria as key variables for discrimination of DMH-treated rats from controls. Real-time PCR confirmed the significant increase of the Ruminococcus obeum and the Allobaculum-like bacteria in DMH-treated rats. UniFrac analysis based on V3 pyrosequencing further validated that the gut microbiota structures of treated and control animals were similar at an early stage, but segregated after ACF formation. Thirteen operational taxonomic units including Ruminococcus-like and Allobaculum-like bacteria were identified as key variables for the discrimination of DMH-treated rats from controls. Dynamic analysis of gut microbiota may become a noninvasive strategy for monitoring host health changes induced by carcinogen exposure.

AB - This study monitored structural shifts of gut microbiota of rats developing precancerous mucosal lesions induced by carcinogen 1,2-dimethyl hydrazine (DMH) treatment using PCR-denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing on the 16S rRNA gene V3 region. Partial least square discriminant analysis of DGGE fingerprints showed that the gut microbiota structure of treated animals was similar to that of the controls 1 and 3 weeks after DMH treatments, but significantly different 7 weeks after DMH treatments, when a large number of aberrant crypt foci (ACF) developed in their colons. Martens' uncertainty test, followed by anova test (P<0.05) identified Ruminococcus-like and Allobaculum-like bacteria as key variables for discrimination of DMH-treated rats from controls. Real-time PCR confirmed the significant increase of the Ruminococcus obeum and the Allobaculum-like bacteria in DMH-treated rats. UniFrac analysis based on V3 pyrosequencing further validated that the gut microbiota structures of treated and control animals were similar at an early stage, but segregated after ACF formation. Thirteen operational taxonomic units including Ruminococcus-like and Allobaculum-like bacteria were identified as key variables for the discrimination of DMH-treated rats from controls. Dynamic analysis of gut microbiota may become a noninvasive strategy for monitoring host health changes induced by carcinogen exposure.

KW - colorectal cancer

KW - gut microbiota

KW - health assessment

UR - http://www.scopus.com/inward/record.url?scp=77955238861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955238861&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6941.2010.00924.x

DO - 10.1111/j.1574-6941.2010.00924.x

M3 - Article

C2 - 20629751

AN - SCOPUS:77955238861

VL - 73

SP - 577

EP - 586

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 3

ER -