TY - JOUR
T1 - Structural perturbation of superfolder GFP in the presence of guanidine thiocyanate
AU - Stepanenko, Olesya V.
AU - Stepanenko, Olga V.
AU - Kuznetsova, Irina M.
AU - Verkhusha, Vladyslav V.
AU - Turoverov, Konstantin K.
PY - 2012
Y1 - 2012
N2 - The guanidine-thiocyanate-induced denaturation-renaturation of sfGFP was studied. It was shown that the disruption of sfGFP native structure occurs in the range of guanidine thiocyanate concentrations from 0.5 to 2.5 M. This process was accompanied by simultaneous changes of all recorded parameters. It was found that the small guanidine thiocyanate concentrations (less then 0.1-0.2 M) triggered local structural disturbances in protein which result in significant decrease of chromophore and tryptophan fluorescence intensity and change of protein visible absorption spectrum.
AB - The guanidine-thiocyanate-induced denaturation-renaturation of sfGFP was studied. It was shown that the disruption of sfGFP native structure occurs in the range of guanidine thiocyanate concentrations from 0.5 to 2.5 M. This process was accompanied by simultaneous changes of all recorded parameters. It was found that the small guanidine thiocyanate concentrations (less then 0.1-0.2 M) triggered local structural disturbances in protein which result in significant decrease of chromophore and tryptophan fluorescence intensity and change of protein visible absorption spectrum.
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U2 - 10.1155/2012/868154
DO - 10.1155/2012/868154
M3 - Article
AN - SCOPUS:84866163804
SN - 0712-4813
VL - 27
SP - 381
EP - 386
JO - Spectroscopy (New York)
JF - Spectroscopy (New York)
IS - 5-6
ER -