Structural perturbation of super-folder GFP in the presence of guanidine thiocyanate

Olesya V. Stepanenko; Olga V. Stepanenko; Irina M. Kuznetsova; Vladislav V. Verkhusha; Konstantin K. Turoverov

doi:10.3233/978-1-61499-184-7-249

Structural perturbation of super-folder GFP in the presence of guanidine thiocyanate

Olesya V. Stepanenko, Olga V. Stepanenko, Irina M. Kuznetsova, Vladislav V. Verkhusha, Konstantin K. Turoverov

Anatomy & Structural Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The guanidine thiocyanate induced denaturation-renaturation of sfGFP was studied. It was shown that the disruption of sfGFP native structure occurs in the range of guanidine thiocyanate concentrations from 0.5 to 2.5 M. This process was accompanied by simultaneous changes of all recorded parameters. It was found that the small guanidine thiocyanate concentrations (less then 0.1-0.2 M) triggered local structural disturbances in protein which result in significant decrease of chromophore and tryptophan fluorescence intensity and change of protein visible absorption spectrum.

Original language	English (US)
Title of host publication	Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011
Editors	Maria Marques, Luis Batista de Carvalho, Parvez Haris
Pages	249-254
Number of pages	6
DOIs	https://doi.org/10.3233/978-1-61499-184-7-249
State	Published - Nov 27 2013

Publication series

Name	Advances in Biomedical Spectroscopy
Volume	7
ISSN (Print)	1875-0656

Keywords

Fluorescent proteins
folding of proteins with beta-barrel topology
small guanidine thiocyanate concentrations
super-folder GFP

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

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10.3233/978-1-61499-184-7-249

Cite this

Stepanenko, O. V., Stepanenko, O. V., Kuznetsova, I. M., Verkhusha, V. V., & Turoverov, K. K. (2013). Structural perturbation of super-folder GFP in the presence of guanidine thiocyanate. In M. Marques, L. Batista de Carvalho, & P. Haris (Eds.), Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011 (pp. 249-254). (Advances in Biomedical Spectroscopy; Vol. 7). https://doi.org/10.3233/978-1-61499-184-7-249

Structural perturbation of super-folder GFP in the presence of guanidine thiocyanate. / Stepanenko, Olesya V.; Stepanenko, Olga V.; Kuznetsova, Irina M. et al.
Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011. ed. / Maria Marques; Luis Batista de Carvalho; Parvez Haris. 2013. p. 249-254 (Advances in Biomedical Spectroscopy; Vol. 7).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Stepanenko, OV, Stepanenko, OV, Kuznetsova, IM, Verkhusha, VV & Turoverov, KK 2013, Structural perturbation of super-folder GFP in the presence of guanidine thiocyanate. in M Marques, L Batista de Carvalho & P Haris (eds), Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011. Advances in Biomedical Spectroscopy, vol. 7, pp. 249-254. https://doi.org/10.3233/978-1-61499-184-7-249

Stepanenko OV, Stepanenko OV, Kuznetsova IM, Verkhusha VV, Turoverov KK. Structural perturbation of super-folder GFP in the presence of guanidine thiocyanate. In Marques M, Batista de Carvalho L, Haris P, editors, Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011. 2013. p. 249-254. (Advances in Biomedical Spectroscopy). doi: 10.3233/978-1-61499-184-7-249

Stepanenko, Olesya V. ; Stepanenko, Olga V. ; Kuznetsova, Irina M. et al. / Structural perturbation of super-folder GFP in the presence of guanidine thiocyanate. Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011. editor / Maria Marques ; Luis Batista de Carvalho ; Parvez Haris. 2013. pp. 249-254 (Advances in Biomedical Spectroscopy).

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abstract = "The guanidine thiocyanate induced denaturation-renaturation of sfGFP was studied. It was shown that the disruption of sfGFP native structure occurs in the range of guanidine thiocyanate concentrations from 0.5 to 2.5 M. This process was accompanied by simultaneous changes of all recorded parameters. It was found that the small guanidine thiocyanate concentrations (less then 0.1-0.2 M) triggered local structural disturbances in protein which result in significant decrease of chromophore and tryptophan fluorescence intensity and change of protein visible absorption spectrum.",

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Structural perturbation of super-folder GFP in the presence of guanidine thiocyanate

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