TY - JOUR
T1 - Structural determinants for the formation of sulfhemeprotein complexes
AU - Román-Morales, Elddie
AU - Pietri, Ruth
AU - Ramos-Santana, Brenda
AU - Vinogradov, Serge N.
AU - Lewis-Ballester, Ariel
AU - López-Garriga, Juan
N1 - Funding Information:
This work was supported in part by funds from the National Science Foundation (Grant 0843608 ) and NIH-NIGMS/MBRS-SCORE 5 S06GM008103-36. We thank Dr. Syun-Ru Yeh for the resonance Raman facilities and Dr. Carmen Cadilla for the construction of the HbI mutants. Special thank to Dr. Jack Peisach for his motivation and his inspiring work on sulfheme proteins. We also thank graduate and undergraduate students Cacimar Ramos, Laura Granell, Tatiana Quiñones, Darya Marchany and Frances Marie Pietri for their assistance during the work.
PY - 2010/10/1
Y1 - 2010/10/1
N2 - Several hemoglobins were explored by UV-Vis and resonance Raman spectroscopy to define sulfheme complex formation. Evaluation of these proteins upon the reaction with H 2O 2 or O 2 in the presence of H 2S suggest: (a) the formation of the sulfheme derivate requires a HisE7 residue in the heme distal site with an adequate orientation to form an active ternary complex; (b) that the ternary complex intermediate involves the HisE7, the peroxo or ferryl species, and the H 2S molecule. This moiety precedes and triggers the sulfheme formation.
AB - Several hemoglobins were explored by UV-Vis and resonance Raman spectroscopy to define sulfheme complex formation. Evaluation of these proteins upon the reaction with H 2O 2 or O 2 in the presence of H 2S suggest: (a) the formation of the sulfheme derivate requires a HisE7 residue in the heme distal site with an adequate orientation to form an active ternary complex; (b) that the ternary complex intermediate involves the HisE7, the peroxo or ferryl species, and the H 2S molecule. This moiety precedes and triggers the sulfheme formation.
KW - Ferryl species
KW - Hemoglobin I (HbI)
KW - Histidine (His)
KW - Hydrogen peroxide (H O )
KW - Hydrogen sulfide (H S)
KW - Sulfhemoglobin
KW - Sulfmyoglobin
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U2 - 10.1016/j.bbrc.2010.08.068
DO - 10.1016/j.bbrc.2010.08.068
M3 - Article
C2 - 20732304
AN - SCOPUS:77957284966
SN - 0006-291X
VL - 400
SP - 489
EP - 492
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -