Structural characterization of the fusion of two pentapeptide repeat proteins, Np275 and Np276, from Nostoc punctiforme: Resurrection of an ancestral protein

Matthew W. Vetting, Subray Hegde, Keith Z. Hazleton, John S. Blanchard

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The Nostoc punctiforme genes Np275 and Np276 are two adjacently encoded proteins of 98 and 75 amino acids in length and exhibit sequences composed of tandem pentapeptide repeats. The structures of Np275 and a fusion of Np275 and Np276 were determined to 2.1 and 1.5 Å , respectively. The two Nostoc proteins fold as highly symmetric right-handed quadrilateral β-helices similar to the mycobacterial protein MfpA implicated in fluoroquinolone resistance and DNA gyrase inhibition. The sequence composition of the intervening coding region and the ability to express a fused protein by removing the stop codon for Np275 suggests Np275 and Np276 were recently part of a larger ancestral pentapeptide repeat protein. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)755-760
Number of pages6
JournalProtein Science
Volume16
Issue number4
DOIs
StatePublished - Apr 2007

Fingerprint

Nostoc
Fusion reactions
Proteins
DNA Gyrase
Tandem Repeat Sequences
Terminator Codon
Fluoroquinolones
Ports and harbors
Introns
Genes
Amino Acids
Chemical analysis

Keywords

  • Cyanobacterium
  • Fusion protein
  • Pentapeptide repeat protein
  • Right-handed quadrilateral β-helix
  • Structure determination

ASJC Scopus subject areas

  • Biochemistry

Cite this

@article{0d51b1bdf06c4c1ea502c127ca856f51,
title = "Structural characterization of the fusion of two pentapeptide repeat proteins, Np275 and Np276, from Nostoc punctiforme: Resurrection of an ancestral protein",
abstract = "The Nostoc punctiforme genes Np275 and Np276 are two adjacently encoded proteins of 98 and 75 amino acids in length and exhibit sequences composed of tandem pentapeptide repeats. The structures of Np275 and a fusion of Np275 and Np276 were determined to 2.1 and 1.5 {\AA} , respectively. The two Nostoc proteins fold as highly symmetric right-handed quadrilateral β-helices similar to the mycobacterial protein MfpA implicated in fluoroquinolone resistance and DNA gyrase inhibition. The sequence composition of the intervening coding region and the ability to express a fused protein by removing the stop codon for Np275 suggests Np275 and Np276 were recently part of a larger ancestral pentapeptide repeat protein. Published by Cold Spring Harbor Laboratory Press.",
keywords = "Cyanobacterium, Fusion protein, Pentapeptide repeat protein, Right-handed quadrilateral β-helix, Structure determination",
author = "Vetting, {Matthew W.} and Subray Hegde and Hazleton, {Keith Z.} and Blanchard, {John S.}",
year = "2007",
month = "4",
doi = "10.1110/ps.062637707",
language = "English (US)",
volume = "16",
pages = "755--760",
journal = "Protein Science",
issn = "0961-8368",
publisher = "Cold Spring Harbor Laboratory Press",
number = "4",

}

TY - JOUR

T1 - Structural characterization of the fusion of two pentapeptide repeat proteins, Np275 and Np276, from Nostoc punctiforme

T2 - Resurrection of an ancestral protein

AU - Vetting, Matthew W.

AU - Hegde, Subray

AU - Hazleton, Keith Z.

AU - Blanchard, John S.

PY - 2007/4

Y1 - 2007/4

N2 - The Nostoc punctiforme genes Np275 and Np276 are two adjacently encoded proteins of 98 and 75 amino acids in length and exhibit sequences composed of tandem pentapeptide repeats. The structures of Np275 and a fusion of Np275 and Np276 were determined to 2.1 and 1.5 Å , respectively. The two Nostoc proteins fold as highly symmetric right-handed quadrilateral β-helices similar to the mycobacterial protein MfpA implicated in fluoroquinolone resistance and DNA gyrase inhibition. The sequence composition of the intervening coding region and the ability to express a fused protein by removing the stop codon for Np275 suggests Np275 and Np276 were recently part of a larger ancestral pentapeptide repeat protein. Published by Cold Spring Harbor Laboratory Press.

AB - The Nostoc punctiforme genes Np275 and Np276 are two adjacently encoded proteins of 98 and 75 amino acids in length and exhibit sequences composed of tandem pentapeptide repeats. The structures of Np275 and a fusion of Np275 and Np276 were determined to 2.1 and 1.5 Å , respectively. The two Nostoc proteins fold as highly symmetric right-handed quadrilateral β-helices similar to the mycobacterial protein MfpA implicated in fluoroquinolone resistance and DNA gyrase inhibition. The sequence composition of the intervening coding region and the ability to express a fused protein by removing the stop codon for Np275 suggests Np275 and Np276 were recently part of a larger ancestral pentapeptide repeat protein. Published by Cold Spring Harbor Laboratory Press.

KW - Cyanobacterium

KW - Fusion protein

KW - Pentapeptide repeat protein

KW - Right-handed quadrilateral β-helix

KW - Structure determination

UR - http://www.scopus.com/inward/record.url?scp=33947721335&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947721335&partnerID=8YFLogxK

U2 - 10.1110/ps.062637707

DO - 10.1110/ps.062637707

M3 - Article

C2 - 17384236

AN - SCOPUS:33947721335

VL - 16

SP - 755

EP - 760

JO - Protein Science

JF - Protein Science

SN - 0961-8368

IS - 4

ER -