Structural characterization of oxidized dimeric Scapharca inaequivalvis hemoglobin by resonance Raman spectroscopy

Alberto Boffi, Satoshi Takahashi, Carla Spagnuolo, Denis L. Rousseau, Emilia Chiancone

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Resonance Raman spectra of the ferric homodimeric hemoglobin from Scapharca inaequivalvis have been measured over the pH range 5.8-8.3 in buffers of ionic strengths 0.01 and 0.1 M to determine the spin and coordination state of the iron atom. Three species contribute to the spectra: a low spin hexacoordinate, a high spin pentacoordinate, and a high spin hexacoordinate component. Optical absorption and EPR spectra measured under the same conditions allowed the identification of the ligands in the sixth coordination position, namely the distal histidine in the low spin derivative and a water molecule in the high spin one. The relative concentrations of these three species depend on pH in an unusual way. Thus, the aquomet derivative is present over the whole pH range, albeit in small amounts as most of the hemoglobin converts to the low spin hemichrome at acid pH values and to the pentacoordinate derivative at neutral and slightly alkaline ones. The formation of a pentacoordinate heme as the pH is increased has not been reported previously for other myoglobins and hemoglobins. Low ionic strength and high protein concentration favor the formation of the high spin pentacoordinate species, while at high ionic strength and low protein concentration the low spin hexacoordinate species prevails. Ionization of the iron-bound water molecule occurs at pH ≥ 9.3; accordingly, signals from the hydroxyl derivative were not observed in the Raman spectra over the pH range studied.

Original languageEnglish (US)
Pages (from-to)20437-20440
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number32
StatePublished - Aug 12 1994
Externally publishedYes

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Raman Spectrum Analysis
Raman spectroscopy
Ionic strength
Derivatives
Hemoglobins
Raman scattering
Osmolar Concentration
Iron
Molecules
Water
Myoglobin
Scapharca
Heme
Histidine
Hydroxyl Radical
Light absorption
Ionization
Paramagnetic resonance
Buffers
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structural characterization of oxidized dimeric Scapharca inaequivalvis hemoglobin by resonance Raman spectroscopy. / Boffi, Alberto; Takahashi, Satoshi; Spagnuolo, Carla; Rousseau, Denis L.; Chiancone, Emilia.

In: Journal of Biological Chemistry, Vol. 269, No. 32, 12.08.1994, p. 20437-20440.

Research output: Contribution to journalArticle

Boffi, Alberto ; Takahashi, Satoshi ; Spagnuolo, Carla ; Rousseau, Denis L. ; Chiancone, Emilia. / Structural characterization of oxidized dimeric Scapharca inaequivalvis hemoglobin by resonance Raman spectroscopy. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 32. pp. 20437-20440.
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