Structural changes at the heme induced by freezing hemoglobin

M. R. Ondrias, Denis L. Rousseau, S. R. Simon

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A dramatic change occurs in the vibrational properties of the iron-histidine bond, trans to the oxygen binding site, on freezing deoxyhemoglobin. The large, quaternary structure-dependent differences in the shape and frequency of the iron-histidine mode observed in resonance Raman scattering measurements above freezing are significantly diminished by the freezing event and the scattering intensity increases substantially. On further reduction in temperature to 10 K this broad line becomes narrow and shifts to a higher frequency. These data implicate dynamical processes and protein interaction with water as contributors to the quaternary structure dependence of the iron-histidine bond and thus reflect on the role of that bond in the energetics of cooperative ligand binding.

Original languageEnglish (US)
Pages (from-to)657-659
Number of pages3
JournalScience
Volume213
Issue number4508
StatePublished - 1981
Externally publishedYes

Fingerprint

Heme
Histidine
Freezing
Hemoglobins
Iron
Raman Spectrum Analysis
Binding Sites
Oxygen
Ligands
Temperature
Water
Proteins

ASJC Scopus subject areas

  • General

Cite this

Ondrias, M. R., Rousseau, D. L., & Simon, S. R. (1981). Structural changes at the heme induced by freezing hemoglobin. Science, 213(4508), 657-659.

Structural changes at the heme induced by freezing hemoglobin. / Ondrias, M. R.; Rousseau, Denis L.; Simon, S. R.

In: Science, Vol. 213, No. 4508, 1981, p. 657-659.

Research output: Contribution to journalArticle

Ondrias, MR, Rousseau, DL & Simon, SR 1981, 'Structural changes at the heme induced by freezing hemoglobin', Science, vol. 213, no. 4508, pp. 657-659.
Ondrias, M. R. ; Rousseau, Denis L. ; Simon, S. R. / Structural changes at the heme induced by freezing hemoglobin. In: Science. 1981 ; Vol. 213, No. 4508. pp. 657-659.
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