Structural and functional vocal fold epithelial integrity following injury

Ciara Leydon, Mitsuyoshi Imaizumi, David Yang, Susan L. Thibeault, Marvin P. Fried

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives/Hypothesis: An intact epithelium is an important part of vocal fold defense. Damage to the epithelium can compromise vocal fold homeostasis and protection of the host tissue from viral and bacterial invasion. Elucidating the effects of damage on epithelial architectural and barrier integrity provides insight into the role of epithelium in protecting vocal folds. Using an animal model, we evaluated the time course of structural and functional epithelial restoration following injury.

Study Design: Prospective, controlled animal study.

Methods: Forty rats underwent surgery to remove vocal fold mucosa unilaterally. Larynges were harvested at five time intervals between 3 to 90 days postinjury and were prepared for histological and permeability analyses.

Results: Rapid restoration of structural integrity was demonstrated by return of a multilayerd epithelium, intercellular junctions, and basement membrane at 5 days postinjury. Atypical epithelial permeability was observed up to 5 weeks postinjury.

Conclusion: Restoration of epithelial barrier integrity lags epithelial structural restoration. Consequently, epithelial regeneration cannot be equated with return of functional barrier integrity. Rather, ongoing leakiness of regenerated epithelium indicates that vocal folds remain at risk for damage, pathogen invasion, and remodeling postinjury.

Original languageEnglish (US)
Pages (from-to)2764-2769
Number of pages6
JournalLaryngoscope
Volume124
Issue number12
DOIs
StatePublished - Dec 1 2014

Fingerprint

Vocal Cords
Epithelium
Wounds and Injuries
Permeability
Architectural Accessibility
Intercellular Junctions
Larynx
Basement Membrane
Regeneration
Mucous Membrane
Homeostasis
Animal Models
Prospective Studies

Keywords

  • Basement membrane
  • Epithelium
  • Permeability
  • Surgery
  • Vocal fold

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Medicine(all)

Cite this

Structural and functional vocal fold epithelial integrity following injury. / Leydon, Ciara; Imaizumi, Mitsuyoshi; Yang, David; Thibeault, Susan L.; Fried, Marvin P.

In: Laryngoscope, Vol. 124, No. 12, 01.12.2014, p. 2764-2769.

Research output: Contribution to journalArticle

Leydon, C, Imaizumi, M, Yang, D, Thibeault, SL & Fried, MP 2014, 'Structural and functional vocal fold epithelial integrity following injury', Laryngoscope, vol. 124, no. 12, pp. 2764-2769. https://doi.org/10.1002/lary.24818
Leydon, Ciara ; Imaizumi, Mitsuyoshi ; Yang, David ; Thibeault, Susan L. ; Fried, Marvin P. / Structural and functional vocal fold epithelial integrity following injury. In: Laryngoscope. 2014 ; Vol. 124, No. 12. pp. 2764-2769.
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