Structural and Functional Studies on the Marburg Virus GP2 Fusion Loop

Nina Liu, Yisong Tao, Michael D. Brenowitz, Mark E. Girvin, Jonathan R. Lai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Marburg virus (MARV) and the ebolaviruses belong to the family Filoviridae (the members of which are filoviruses) that cause severe hemorrhagic fever. Infection requires fusion of the host and viral membranes, a process that occurs in the host cell endosomal compartment and is facilitated by the envelope glycoprotein fusion subunit, GP2. The N-terminal fusion loop (FL) of GP2 is a hydrophobic disulfide-bonded loop that is postulated to insert and disrupt the host endosomal membrane during fusion. Here, we describe the first structural and functional studies of a protein corresponding to the MARV GP2 FL. We found that this protein undergoes a pH-dependent conformational change, as monitored by circular dichroism and nuclear magnetic resonance. Furthermore, we report that, under low pH conditions, the MARV GP2 FL can induce content leakage from liposomes. The general aspects of this pH-dependent structure and lipid-perturbing behavior are consistent with previous reports on Ebola virus GP2 FL. However, nuclear magnetic resonance studies in lipid bicelles and mutational analysis indicate differences in structure exist between MARV and Ebola virus GP2 FL. These results provide new insight into the mechanism of MARV GP2-mediated cell entry.

Original languageEnglish (US)
Pages (from-to)S146-S153
JournalJournal of Infectious Diseases
Volume212
DOIs
StatePublished - Oct 1 2015

Fingerprint

Marburgvirus
Ebolavirus
Filoviridae
Magnetic Resonance Spectroscopy
Lipids
Virus Internalization
Membrane Fusion
Circular Dichroism
Liposomes
Disulfides
Glycoproteins
Proteins
Fever
Infection

Keywords

  • envelope glycoprotein
  • Marburg virus
  • viral entry
  • viral membrane fusion

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Structural and Functional Studies on the Marburg Virus GP2 Fusion Loop. / Liu, Nina; Tao, Yisong; Brenowitz, Michael D.; Girvin, Mark E.; Lai, Jonathan R.

In: Journal of Infectious Diseases, Vol. 212, 01.10.2015, p. S146-S153.

Research output: Contribution to journalArticle

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