Structural and biochemical characterization of Chlamydia trachomatis hypothetical protein CT263 supports that menaquinone synthesis occurs through the futalosine pathway

Michael L. Barta, Keisha Thomas, Hongling Yuan, Scott Lovell, Kevin P. Battaile, Vern L. Schramm, P. Scott Hefty

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The obligate intracellular human pathogen Chlamydia trachomatis is the etiological agent of blinding trachoma and sexually transmitted disease. Genomic sequencing of Chlamydia indicated this medically important bacterium was not exclusively dependent on the host cell for energy. In order for the electron transport chain to function, electron shuttling between membrane-embedded complexes requires lipid-soluble quinones (e.g. menaquionone or ubiquinone). The sources or biosynthetic pathways required to obtain these electron carriers within C. trachomatis are poorly understood. The 1.58Å crystal structure of C. trachomatis hypothetical protein CT263 presented here supports a role in quinone biosynthesis. Although CT263 lacks sequence-based functional annotation, the crystal structure of CT263 displays striking structural similarity to 5'-methylthioadenosine nucleosidase (MTAN) enzymes. Although CT263 lacks the active site-associated dimer interface found in prototypical MTANs, co-crystal structures with product (adenine) or substrate (5'-methylthioadenosine) indicate that the canonical active site residues are conserved. Enzymatic characterization of CT263 indicates that the futalosine pathway intermediate 6-amino-6-deoxyfutalosine (kcat/Km = 1.8 × 103 M-1 s-1), but not the prototypicalMTANsubstrates (e.g. S-adenosylhomocysteine and 5'-methylthioadenosine), is hydrolyzed. Bioinformatic analyses of the chlamydial proteome also support the futalosine pathway toward the synthesis of menaquinone in Chlamydiaceae. This report provides the first experimental support for quinone synthesis in Chlamydia. Menaquinone synthesis provides another target for agents to combat C. trachomatis infection.

Original languageEnglish (US)
Pages (from-to)32214-32229
Number of pages16
JournalJournal of Biological Chemistry
Volume289
Issue number46
DOIs
StatePublished - Nov 14 2014

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Vitamin K 2
Chlamydia trachomatis
Crystal structure
Chlamydia
S-Adenosylhomocysteine
Chlamydiaceae
Catalytic Domain
Quinones
Proteins
Ubiquinone
Electrons
Biosynthesis
Pathogens
Adenine
Proteome
Bioinformatics
Trachoma
Dimers
Chlamydia Infections
Biosynthetic Pathways

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Structural and biochemical characterization of Chlamydia trachomatis hypothetical protein CT263 supports that menaquinone synthesis occurs through the futalosine pathway. / Barta, Michael L.; Thomas, Keisha; Yuan, Hongling; Lovell, Scott; Battaile, Kevin P.; Schramm, Vern L.; Hefty, P. Scott.

In: Journal of Biological Chemistry, Vol. 289, No. 46, 14.11.2014, p. 32214-32229.

Research output: Contribution to journalArticle

Barta, Michael L. ; Thomas, Keisha ; Yuan, Hongling ; Lovell, Scott ; Battaile, Kevin P. ; Schramm, Vern L. ; Hefty, P. Scott. / Structural and biochemical characterization of Chlamydia trachomatis hypothetical protein CT263 supports that menaquinone synthesis occurs through the futalosine pathway. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 46. pp. 32214-32229.
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