TY - JOUR
T1 - Structural analysis of α-carrageenan sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID
AU - Aguilan, Jennifer T.
AU - Dayrit, Fabian M.
AU - Zhang, Jinhua
AU - Niñonuevo, Milady R.
AU - Lebrilla, Carlito B.
N1 - Funding Information:
This work was partially supported by the Department of Science and Technology, Philippines. The authors thank the University of California, Davis, and the Ateneo de Manila University for providing the funding for the short-term exchange visitor scholarship program for JTA. CBL thanks the NIH (USA) for its continued support.
PY - 2006/1
Y1 - 2006/1
N2 - Structural analysis of sulfated oligosaccharides from κ-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.
AB - Structural analysis of sulfated oligosaccharides from κ-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.
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U2 - 10.1016/j.jasms.2005.09.009
DO - 10.1016/j.jasms.2005.09.009
M3 - Article
C2 - 16352442
AN - SCOPUS:29744432247
SN - 1044-0305
VL - 17
SP - 96
EP - 103
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 1
ER -