Stromal osteonectin overexpression is associated with poor outcome in patients with ampullary cancer

Mark Bloomston, E. Christopher Ellison, Peter Muscarella, Osama Al-Saif, Edward W. Martin, W. Scott Melvin, Wendy L. Frankel

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Osteonectin has been suggested to be important in the progression of pancreatic cancer but has not been correlated with survival. We determined the osteonectin expression and its influence on survival in patients with ampullary carcinoma. Methods: Tissue microarrays were constructed from the tumors of 56 patients with ampullary cancer undergoing pancreaticoduodenectomy. Immunohistochemical staining for osteonectin was undertaken and compared with staining in chronic pancreatitis (n = 13) and normal pancreas (n = 19). Survival curves were created by the Kaplan-Meier method and compared by log rank analysis. Median follow-up for all living patients with ampullary cancer was 69.6 months. Results: Osteonectin was significantly (P < .05, Fisher's exact test) overexpressed in the stroma of ampullary cancers (90%) relative to chronic pancreatitis (62%) and normal pancreas (0%). Tumors expressing osteonectin were more likely to have nodal metastases than those lacking osteonectin expression (48% vs. 0%, P = .06, Fisher's exact test) and showed decreased survival. Node-negative status, pylorus preservation at the time of pancreaticoduodenectomy, and lack of osteonectin expression were predictors of prolonged survival by multivariate analysis. Conclusions: Although the importance of tumor-stroma interactions in periampullary cancers is not fully understood, our data suggest that osteonectin is an integral stromal element in ampullary cancers, and its overexpression is associated with decreased survival.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalAnnals of Surgical Oncology
Volume14
Issue number1
DOIs
StatePublished - Jan 2007
Externally publishedYes

Fingerprint

Osteonectin
Neoplasms
Survival
Pancreaticoduodenectomy
Chronic Pancreatitis
Pancreas
Staining and Labeling
Pylorus
Survival Analysis
Pancreatic Neoplasms
Multivariate Analysis
Neoplasm Metastasis
Carcinoma

Keywords

  • Ampulla of Vater
  • Ampullary cancer
  • Osteonectin
  • Osteopontin
  • Survival
  • Tissue microarray

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Stromal osteonectin overexpression is associated with poor outcome in patients with ampullary cancer. / Bloomston, Mark; Ellison, E. Christopher; Muscarella, Peter; Al-Saif, Osama; Martin, Edward W.; Melvin, W. Scott; Frankel, Wendy L.

In: Annals of Surgical Oncology, Vol. 14, No. 1, 01.2007, p. 211-217.

Research output: Contribution to journalArticle

Bloomston, Mark ; Ellison, E. Christopher ; Muscarella, Peter ; Al-Saif, Osama ; Martin, Edward W. ; Melvin, W. Scott ; Frankel, Wendy L. / Stromal osteonectin overexpression is associated with poor outcome in patients with ampullary cancer. In: Annals of Surgical Oncology. 2007 ; Vol. 14, No. 1. pp. 211-217.
@article{22f74e97bb6e4e9f956e1507c3a77819,
title = "Stromal osteonectin overexpression is associated with poor outcome in patients with ampullary cancer",
abstract = "Background: Osteonectin has been suggested to be important in the progression of pancreatic cancer but has not been correlated with survival. We determined the osteonectin expression and its influence on survival in patients with ampullary carcinoma. Methods: Tissue microarrays were constructed from the tumors of 56 patients with ampullary cancer undergoing pancreaticoduodenectomy. Immunohistochemical staining for osteonectin was undertaken and compared with staining in chronic pancreatitis (n = 13) and normal pancreas (n = 19). Survival curves were created by the Kaplan-Meier method and compared by log rank analysis. Median follow-up for all living patients with ampullary cancer was 69.6 months. Results: Osteonectin was significantly (P < .05, Fisher's exact test) overexpressed in the stroma of ampullary cancers (90{\%}) relative to chronic pancreatitis (62{\%}) and normal pancreas (0{\%}). Tumors expressing osteonectin were more likely to have nodal metastases than those lacking osteonectin expression (48{\%} vs. 0{\%}, P = .06, Fisher's exact test) and showed decreased survival. Node-negative status, pylorus preservation at the time of pancreaticoduodenectomy, and lack of osteonectin expression were predictors of prolonged survival by multivariate analysis. Conclusions: Although the importance of tumor-stroma interactions in periampullary cancers is not fully understood, our data suggest that osteonectin is an integral stromal element in ampullary cancers, and its overexpression is associated with decreased survival.",
keywords = "Ampulla of Vater, Ampullary cancer, Osteonectin, Osteopontin, Survival, Tissue microarray",
author = "Mark Bloomston and Ellison, {E. Christopher} and Peter Muscarella and Osama Al-Saif and Martin, {Edward W.} and Melvin, {W. Scott} and Frankel, {Wendy L.}",
year = "2007",
month = "1",
doi = "10.1245/s10434-006-9128-3",
language = "English (US)",
volume = "14",
pages = "211--217",
journal = "Annals of Surgical Oncology",
issn = "1068-9265",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Stromal osteonectin overexpression is associated with poor outcome in patients with ampullary cancer

AU - Bloomston, Mark

AU - Ellison, E. Christopher

AU - Muscarella, Peter

AU - Al-Saif, Osama

AU - Martin, Edward W.

AU - Melvin, W. Scott

AU - Frankel, Wendy L.

PY - 2007/1

Y1 - 2007/1

N2 - Background: Osteonectin has been suggested to be important in the progression of pancreatic cancer but has not been correlated with survival. We determined the osteonectin expression and its influence on survival in patients with ampullary carcinoma. Methods: Tissue microarrays were constructed from the tumors of 56 patients with ampullary cancer undergoing pancreaticoduodenectomy. Immunohistochemical staining for osteonectin was undertaken and compared with staining in chronic pancreatitis (n = 13) and normal pancreas (n = 19). Survival curves were created by the Kaplan-Meier method and compared by log rank analysis. Median follow-up for all living patients with ampullary cancer was 69.6 months. Results: Osteonectin was significantly (P < .05, Fisher's exact test) overexpressed in the stroma of ampullary cancers (90%) relative to chronic pancreatitis (62%) and normal pancreas (0%). Tumors expressing osteonectin were more likely to have nodal metastases than those lacking osteonectin expression (48% vs. 0%, P = .06, Fisher's exact test) and showed decreased survival. Node-negative status, pylorus preservation at the time of pancreaticoduodenectomy, and lack of osteonectin expression were predictors of prolonged survival by multivariate analysis. Conclusions: Although the importance of tumor-stroma interactions in periampullary cancers is not fully understood, our data suggest that osteonectin is an integral stromal element in ampullary cancers, and its overexpression is associated with decreased survival.

AB - Background: Osteonectin has been suggested to be important in the progression of pancreatic cancer but has not been correlated with survival. We determined the osteonectin expression and its influence on survival in patients with ampullary carcinoma. Methods: Tissue microarrays were constructed from the tumors of 56 patients with ampullary cancer undergoing pancreaticoduodenectomy. Immunohistochemical staining for osteonectin was undertaken and compared with staining in chronic pancreatitis (n = 13) and normal pancreas (n = 19). Survival curves were created by the Kaplan-Meier method and compared by log rank analysis. Median follow-up for all living patients with ampullary cancer was 69.6 months. Results: Osteonectin was significantly (P < .05, Fisher's exact test) overexpressed in the stroma of ampullary cancers (90%) relative to chronic pancreatitis (62%) and normal pancreas (0%). Tumors expressing osteonectin were more likely to have nodal metastases than those lacking osteonectin expression (48% vs. 0%, P = .06, Fisher's exact test) and showed decreased survival. Node-negative status, pylorus preservation at the time of pancreaticoduodenectomy, and lack of osteonectin expression were predictors of prolonged survival by multivariate analysis. Conclusions: Although the importance of tumor-stroma interactions in periampullary cancers is not fully understood, our data suggest that osteonectin is an integral stromal element in ampullary cancers, and its overexpression is associated with decreased survival.

KW - Ampulla of Vater

KW - Ampullary cancer

KW - Osteonectin

KW - Osteopontin

KW - Survival

KW - Tissue microarray

UR - http://www.scopus.com/inward/record.url?scp=33845634406&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845634406&partnerID=8YFLogxK

U2 - 10.1245/s10434-006-9128-3

DO - 10.1245/s10434-006-9128-3

M3 - Article

VL - 14

SP - 211

EP - 217

JO - Annals of Surgical Oncology

JF - Annals of Surgical Oncology

SN - 1068-9265

IS - 1

ER -