Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion

Akira Orimo, Piyush B. Gupta, Dennis C. Sgroi, Fernando Arenzana-Seisdedos, Thierry Delaunay, Rizwan C. Naeem, Vincent J. Carey, Andrea L. Richardson, Robert A. Weinberg

Research output: Contribution to journalArticle

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Abstract

Fibroblasts often constitute the majority of the stromal cells within a breast carcinoma, yet the functional contributions of these cells to tumorigenesis are poorly understood. Using a coimplantation tumor xenograft model, we demonstrate that carcinoma-associated fibroblasts (CAFs) extracted from human breast carcinomas promote the growth of admixed breast carcinoma cells significantly more than do normal mammary fibroblasts derived from the same patients. The CAFs, which exhibit the traits of myofibroblasts, play a central role in promoting the growth of tumor cells through their ability to secrete stromal cell-derived factor 1 (SDF-1); CAFs promote angiogenesis by recruiting endothelial progenitor cells (EPCs) into carcinomas, an effect mediated in part by SDF-1. CAF-secreted SDF-1 also stimulates tumor growth directly, acting through the cognate receptor, CXCR4, which is expressed by carcinoma cells. Our findings indicate that fibroblasts within invasive breast carcinomas contribute to tumor promotion in large part through the secretion of SDF-1.

Original languageEnglish (US)
Pages (from-to)335-348
Number of pages14
JournalCell
Volume121
Issue number3
DOIs
StatePublished - May 6 2005
Externally publishedYes

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Chemokine CXCL12
Fibroblasts
Tumors
Breast Neoplasms
Carcinoma
Growth
Neoplasms
Cells
CXCR4 Receptors
Myofibroblasts
Endothelial cells
Stromal Cells
Heterografts
Carcinogenesis
Breast

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. / Orimo, Akira; Gupta, Piyush B.; Sgroi, Dennis C.; Arenzana-Seisdedos, Fernando; Delaunay, Thierry; Naeem, Rizwan C.; Carey, Vincent J.; Richardson, Andrea L.; Weinberg, Robert A.

In: Cell, Vol. 121, No. 3, 06.05.2005, p. 335-348.

Research output: Contribution to journalArticle

Orimo, A, Gupta, PB, Sgroi, DC, Arenzana-Seisdedos, F, Delaunay, T, Naeem, RC, Carey, VJ, Richardson, AL & Weinberg, RA 2005, 'Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion', Cell, vol. 121, no. 3, pp. 335-348. https://doi.org/10.1016/j.cell.2005.02.034
Orimo, Akira ; Gupta, Piyush B. ; Sgroi, Dennis C. ; Arenzana-Seisdedos, Fernando ; Delaunay, Thierry ; Naeem, Rizwan C. ; Carey, Vincent J. ; Richardson, Andrea L. ; Weinberg, Robert A. / Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. In: Cell. 2005 ; Vol. 121, No. 3. pp. 335-348.
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