TY - JOUR
T1 - Stretching the timescale of intravital imaging in tumors
AU - Gligorijevic, Bojana
AU - Condeelis, John
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2009
Y1 - 2009
N2 - Since the time it was pioneered in 1992, intravital imaging of tumors at cellular resolution has offered us the extremely important opportunity of "seeing biology". However, until now, most studies were monitoring tumor cell behavior in the same animal over short times, requiring the combining of acquired data into a hypothesis via statistical analysis. In the last year, different groups have independently developed techniques to extend the time scale of intravital imaging to several days. This improvement allows one to address the connection between tumor cell behavior and the microenvironment which surrounds them. We can now assess dynamics of the cell-cell interactions in tumors, analyze tumor cell fate and changes in the tumor extracellular matrix which accompany tumor progression.
AB - Since the time it was pioneered in 1992, intravital imaging of tumors at cellular resolution has offered us the extremely important opportunity of "seeing biology". However, until now, most studies were monitoring tumor cell behavior in the same animal over short times, requiring the combining of acquired data into a hypothesis via statistical analysis. In the last year, different groups have independently developed techniques to extend the time scale of intravital imaging to several days. This improvement allows one to address the connection between tumor cell behavior and the microenvironment which surrounds them. We can now assess dynamics of the cell-cell interactions in tumors, analyze tumor cell fate and changes in the tumor extracellular matrix which accompany tumor progression.
KW - Dorsal skinfold chamber
KW - Intravital
KW - Mammary imaging window
KW - Microenvironment
KW - Multiphoton
KW - Photoswitching
KW - Second harmonic generation
KW - Spinning disc
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U2 - 10.4161/cam.3.4.9581
DO - 10.4161/cam.3.4.9581
M3 - Review article
C2 - 19786830
AN - SCOPUS:85011939871
VL - 3
JO - Cell Adhesion and Migration
JF - Cell Adhesion and Migration
SN - 1933-6918
IS - 4
ER -