Streptomyces thermoautotrophicus does not fix nitrogen

Drew Mackellar, Lucas Lieber, Jeffrey S. Norman, Anthony Bolger, Cory Tobin, James W. Murray, Mehtap Oksaksin, Roger L. Chang, Tyler J. Ford, Peter Q. Nguyen, Jimmy Woodward, Hugo R. Permingeat, Neel S. Joshi, Pamela A. Silver, Björn Usadel, Alfred W. Rutherford, Maren L. Friesen, Jürgen Prell

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Streptomyces thermoautotrophicus UBT1 has been described as a moderately thermophilic chemolithoautotroph with a novel nitrogenase enzyme that is oxygen-insensitive. We have cultured the UBT1 strain, and have isolated two new strains (H1 and P1-2) of very similar phenotypic and genetic characters. These strains show minimal growth on ammonium-free media, and fail to incorporate isotopically labeled N 2 gas into biomass in multiple independent assays. The sdn genes previously published as the putative nitrogenase of S.Thermoautotrophicus have little similarity to anything found in draft genome sequences, published here, for strains H1 and UBT1, but share >99% nucleotide identity with genes from Hydrogenibacillus schlegelii, a draft genome for which is also presented here. H. schlegelii similarly lacks nitrogenase genes and is a non-diazotroph. We propose reclassification of the species containing strains UBT1, H1, and P1-2 as a non-Streptomycete, non-diazotrophic, facultative chemolithoautotroph and conclude that the existence of the previously proposed oxygen-Tolerant nitrogenase is extremely unlikely.

Original languageEnglish (US)
Article number20086
JournalScientific reports
Volume6
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • General

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