Strain-specific determinants on the major phosphoprotein of murine mammary tumor viruses

Janis Racevskis, Nurul H. Sarkar

Research output: Contribution to journalArticle

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Abstract

The major phosphoprotein (p23) of murine mammary tumor virus (MuMTV) was purified for C3H virus, using a combination of alkyl agarose chromatography and gel filtration. Monospecific antiserum was raised against glutaraldehyde crosslinked polymers of purified p23, and the antiserum was then used with iodinated p23 to develop a competition radioimmunoassay. Phosphoprotein p23 was shown to contain both group- and type-specific antigenic determinants in assays using detergent-disrupted virions of MuMTV strains C3H, RIII, GR, and DBA/2 as competing antigens. Tryptic and chymotryptic peptide mapping analysis of p23 polypeptides isolated from MuMTV strains C3H, RIII, GR and DBA/2 showed that the p23s of each virus strain could be clearly distinguished from each other.

Original languageEnglish (US)
Pages (from-to)230-235
Number of pages6
JournalVirology
Volume125
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

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Gammaretrovirus
Phosphoproteins
Breast Neoplasms
Immune Sera
Viruses
Agarose Chromatography
Peptide Mapping
Glutaral
Virion
Detergents
Radioimmunoassay
Gel Chromatography
Epitopes
Polymers
Antigens
Peptides

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Strain-specific determinants on the major phosphoprotein of murine mammary tumor viruses. / Racevskis, Janis; Sarkar, Nurul H.

In: Virology, Vol. 125, No. 1, 1983, p. 230-235.

Research output: Contribution to journalArticle

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