TY - JOUR
T1 - Strain-specific determinants on the major phosphoprotein of murine mammary tumor viruses
AU - Racevskis, Janis
AU - Sarkar, Nurul H.
N1 - Funding Information:
and M. De Ciutiis for ex- This work was supported CA-17129, and CA-08748.
PY - 1983/2
Y1 - 1983/2
N2 - The major phosphoprotein (p23) of murine mammary tumor virus (MuMTV) was purified for C3H virus, using a combination of alkyl agarose chromatography and gel filtration. Monospecific antiserum was raised against glutaraldehyde crosslinked polymers of purified p23, and the antiserum was then used with iodinated p23 to develop a competition radioimmunoassay. Phosphoprotein p23 was shown to contain both group- and type-specific antigenic determinants in assays using detergent-disrupted virions of MuMTV strains C3H, RIII, GR, and DBA/2 as competing antigens. Tryptic and chymotryptic peptide mapping analysis of p23 polypeptides isolated from MuMTV strains C3H, RIII, GR and DBA/2 showed that the p23s of each virus strain could be clearly distinguished from each other.
AB - The major phosphoprotein (p23) of murine mammary tumor virus (MuMTV) was purified for C3H virus, using a combination of alkyl agarose chromatography and gel filtration. Monospecific antiserum was raised against glutaraldehyde crosslinked polymers of purified p23, and the antiserum was then used with iodinated p23 to develop a competition radioimmunoassay. Phosphoprotein p23 was shown to contain both group- and type-specific antigenic determinants in assays using detergent-disrupted virions of MuMTV strains C3H, RIII, GR, and DBA/2 as competing antigens. Tryptic and chymotryptic peptide mapping analysis of p23 polypeptides isolated from MuMTV strains C3H, RIII, GR and DBA/2 showed that the p23s of each virus strain could be clearly distinguished from each other.
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U2 - 10.1016/0042-6822(83)90076-4
DO - 10.1016/0042-6822(83)90076-4
M3 - Article
C2 - 6187126
AN - SCOPUS:0020694134
SN - 0042-6822
VL - 125
SP - 230
EP - 235
JO - Virology
JF - Virology
IS - 1
ER -