Storage characteristics of multiple-donor pooled red blood cells compared to single-donor red blood cell units

Aabhas Mathur, Raquibul Chowdhury, Christopher D. Hillyer, W. Beau Mitchell, Beth H. Shaz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

BACKGROUND: Each unit of blood donated is processed and stored individually resulting in variability in the amount of red blood cells (RBCs) collected, RBC properties, and the 24-hour posttransfusion RBC survivability. As a result, each unit differs in its ability to deliver oxygen and potentially its effects on the recipient. The goal of this study was to investigate the storage of pooled RBCs from multiple donors in comparison to control standard RBC units. STUDY DESIGN AND METHODS: Two units of irradiated, leukoreduced RBCs of same ABO, D, E, C, and K antigen phenotype were collected from each of five donors using apheresis. One unit from each donor was pooled in a 2-L bag and remaining units were used as controls. After being pooled, RBCs were separated in five bags and stored at 4°C along with the controls. Quality indexes were measured on Days 2, 14, and 28 for all the units. RESULTS: Adenosine triphosphate assays for both pooled and controls showed a slight decrease from Day 2 to Day 28 (pooled/control from 5.22/5.24 to 4.35/4.33 µmol/g hemoglobin [Hb]). 2,3-Diphosphoglycerate was successfully rejuvenated for all RBC units on Day 28 (pooled 11.46 µmol/g Hb; control 11.86 µmol/g Hb). The results showed a nonsignificant difference between pooled and control units, with a general trend of lower standard deviation for pooled units when compared to controls. CONCLUSION: Pooled units have reduced unit-to-unit variability. Future exploration of their immunogenicity is required before using pooled units for transfusion.

Original languageEnglish (US)
Pages (from-to)2941-2947
Number of pages7
JournalTransfusion
Volume56
Issue number12
DOIs
StatePublished - Dec 1 2016
Externally publishedYes

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Erythrocytes
Hemoglobins
2,3-Diphosphoglycerate
Dilatation and Curettage
Blood Component Removal
Adenosine Triphosphate
Oxygen
Phenotype

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Storage characteristics of multiple-donor pooled red blood cells compared to single-donor red blood cell units. / Mathur, Aabhas; Chowdhury, Raquibul; Hillyer, Christopher D.; Mitchell, W. Beau; Shaz, Beth H.

In: Transfusion, Vol. 56, No. 12, 01.12.2016, p. 2941-2947.

Research output: Contribution to journalArticle

Mathur, Aabhas ; Chowdhury, Raquibul ; Hillyer, Christopher D. ; Mitchell, W. Beau ; Shaz, Beth H. / Storage characteristics of multiple-donor pooled red blood cells compared to single-donor red blood cell units. In: Transfusion. 2016 ; Vol. 56, No. 12. pp. 2941-2947.
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