Stomatin inhibits pannexin-1-mediated whole-cell currents by interacting with its carboxyl terminal

Haiying Zhan, Craig S. Moore, Bojun Chen, Xin Zhou, Xin Ming Ma, Kumiko Ijichi, Michael V. L. Bennett, Xue Jun Li, Stephen J. Crocker, Zhao Wen Wang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The pannexin-1 (Panx1) channel (often referred to as the Panx1 hemichannel) is a large-conductance channel in the plasma membrane of many mammalian cells. While opening of the channel is potentially detrimental to the cell, little is known about how it is regulated under physiological conditions. Here we show that stomatin inhibited Panx1 channel activity. In transfected HEK-293 cells, stomatin reduced Panx1-mediated whole-cell currents without altering either the total or membrane surface Panx1 protein expression. Stomatin coimmunoprecipitated with full-length Panx1 as well as a Panx1 fragment containing the fourth membrane-spanning domain and the cytosolic carboxyl terminal. The inhibitory effect of stomatin on Panx1-mediated whole-cell currents was abolished by truncating Panx1 at a site in the cytosolic carboxyl terminal. In primary culture of mouse astrocytes, inhibition of endogenous stomatin expression by small interfering RNA enhanced Panx1-mediated outward whole-cell currents. These observations suggest that stomatin may play important roles in astrocytes and other cells by interacting with Panx1 carboxyl terminal to limit channel opening.

Original languageEnglish (US)
Article numbere39489
JournalPLoS One
Volume7
Issue number6
DOIs
StatePublished - Jun 29 2012

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Membranes
Cell membranes
Small Interfering RNA
Cells
cells
astrocytes
Astrocytes
Proteins
HEK293 Cells
small interfering RNA
Cell Membrane
plasma membrane
protein synthesis
mice

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Zhan, H., Moore, C. S., Chen, B., Zhou, X., Ma, X. M., Ijichi, K., ... Wang, Z. W. (2012). Stomatin inhibits pannexin-1-mediated whole-cell currents by interacting with its carboxyl terminal. PLoS One, 7(6), [e39489]. https://doi.org/10.1371/journal.pone.0039489

Stomatin inhibits pannexin-1-mediated whole-cell currents by interacting with its carboxyl terminal. / Zhan, Haiying; Moore, Craig S.; Chen, Bojun; Zhou, Xin; Ma, Xin Ming; Ijichi, Kumiko; Bennett, Michael V. L.; Li, Xue Jun; Crocker, Stephen J.; Wang, Zhao Wen.

In: PLoS One, Vol. 7, No. 6, e39489, 29.06.2012.

Research output: Contribution to journalArticle

Zhan, H, Moore, CS, Chen, B, Zhou, X, Ma, XM, Ijichi, K, Bennett, MVL, Li, XJ, Crocker, SJ & Wang, ZW 2012, 'Stomatin inhibits pannexin-1-mediated whole-cell currents by interacting with its carboxyl terminal', PLoS One, vol. 7, no. 6, e39489. https://doi.org/10.1371/journal.pone.0039489
Zhan, Haiying ; Moore, Craig S. ; Chen, Bojun ; Zhou, Xin ; Ma, Xin Ming ; Ijichi, Kumiko ; Bennett, Michael V. L. ; Li, Xue Jun ; Crocker, Stephen J. ; Wang, Zhao Wen. / Stomatin inhibits pannexin-1-mediated whole-cell currents by interacting with its carboxyl terminal. In: PLoS One. 2012 ; Vol. 7, No. 6.
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