Stoichiometry Regulates Macromolecular Recognition and Supramolecular Assembly: Examples From Lectin-Glycoconjugate Interaction

T. K. Dam, N. Fan, M. L. Talaga, Curtis F. Brewer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lectin-glycoconjugate interactions are essential for numerous cellular and extracellular events. Binding stoichiometry is one of several factors that regulate the initiation and outcome of lectin-mediated biological functions. Therefore, the knowledge of accurate stoichiometry of glycoconjugate binding is pivotal for delineating the molecular mechanism of lectin-dependent processes. Quantitative precipitation and isothermal titration calorimetry can reliably determine the stoichiometry of glycoconjugate recognition by lectins. Stoichiometry plays important roles in affinity enhancement and it influences the structures of lectin-glycoconjugate cross-linked complexes. Correct stoichiometry helped reveal that the binding epitopes (glycans) on multi- and polyvalent glycoconjugates possess decreasing microscopic binding affinities for lectins and the binding of lectin involves an internal diffusion process called "bind and jump." Interestingly, in certain cases, stoichiometry is unable to regulate affinity enhancement, binding thermodynamics, kinetics of cross-linking, and the lattice structures of the cross-linked complexes.

Original languageEnglish (US)
Title of host publicationFluorescent Sensors and Imaging Agents
PublisherElsevier Inc.
Pages161-177
Number of pages17
Volume8
ISBN (Electronic)9780128031988
ISBN (Print)9780128031995
DOIs
StatePublished - Jun 22 2017

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Glycoconjugates
Lectins
Stoichiometry
Calorimetry
Titration
Polysaccharides
Epitopes
Thermodynamics
Kinetics

Keywords

  • Binding
  • Calorimetry
  • Cross-linking
  • Enthalpy
  • Entropy
  • Glycoconjugate
  • Glycoprotein
  • Lectin
  • Mucin
  • Multivalency
  • Polyvalency
  • Scaffold
  • Stoichiometry

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Stoichiometry Regulates Macromolecular Recognition and Supramolecular Assembly : Examples From Lectin-Glycoconjugate Interaction. / Dam, T. K.; Fan, N.; Talaga, M. L.; Brewer, Curtis F.

Fluorescent Sensors and Imaging Agents. Vol. 8 Elsevier Inc., 2017. p. 161-177.

Research output: Chapter in Book/Report/Conference proceedingChapter

Dam, T. K. ; Fan, N. ; Talaga, M. L. ; Brewer, Curtis F. / Stoichiometry Regulates Macromolecular Recognition and Supramolecular Assembly : Examples From Lectin-Glycoconjugate Interaction. Fluorescent Sensors and Imaging Agents. Vol. 8 Elsevier Inc., 2017. pp. 161-177
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