Stimulation of rat adrenal medulla can induce differential changes in the peptide and mRNA levels of chromogranins, neuropeptides and other constituents of chromaffin granules

G. Höfle, R. Weiler, R. Fischer-Colbrie, C. Humpel, A. Laslop, T. Wohlfarter, R. Hogue-Angeletti, A. Saria, P. J. Fleming, H. Winkler

Research output: Contribution to journalArticle

44 Scopus citations


The levels of various components of chromaffin granules were determined in rat adrenals after treatment with several stimulants. After reserpine the levels of calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and chromogranin B but not those of chromogranin A and secretogranin II were elevated. On the other hand, the mRNA of chromogranins A, B and secretogranin II were significantly increased. Treatment with oxotremorine or nicotine (multiple injections for 2 or 3 days) induced analogous changes for peptide and mRNA levels, however, the increases were smaller and for the mRNA less consistent. A single injection of oxotremorine or nicotine raised only the levels of CGRP and NPY and of the NPY mRNA whereas those of the chromogranins and their respective mRNAs remained unaltered. Amongst the membrane proteins only the levels of dopamine β-hydroxylase are increased after prolonged stimulation, whereas those of cytochrome b-561, carboxypeptidase H and synaptin/synaptophysin (SYN) remain unaltered. Thus, the biosynthesis of chromaffin granules can be regulated in quite sophisticated patterns.

Original languageEnglish (US)
Pages (from-to)321-331
Number of pages11
JournalRegulatory Peptides
Issue number3
Publication statusPublished - Feb 26 1991



  • Adrenal medulla
  • Calcitonin gene-related peptide
  • Chromogranin
  • Cytochrome b-561
  • Neuropeptide Y

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

Cite this