Stimulation of rat adrenal medulla can induce differential changes in the peptide and mRNA levels of chromogranins, neuropeptides and other constituents of chromaffin granules

G. Höfle, R. Weiler, R. Fischer-Colbrie, C. Humpel, A. Laslop, T. Wohlfarter, R. Hogue-Angeletti, A. Saria, P. J. Fleming, H. Winkler

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The levels of various components of chromaffin granules were determined in rat adrenals after treatment with several stimulants. After reserpine the levels of calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and chromogranin B but not those of chromogranin A and secretogranin II were elevated. On the other hand, the mRNA of chromogranins A, B and secretogranin II were significantly increased. Treatment with oxotremorine or nicotine (multiple injections for 2 or 3 days) induced analogous changes for peptide and mRNA levels, however, the increases were smaller and for the mRNA less consistent. A single injection of oxotremorine or nicotine raised only the levels of CGRP and NPY and of the NPY mRNA whereas those of the chromogranins and their respective mRNAs remained unaltered. Amongst the membrane proteins only the levels of dopamine β-hydroxylase are increased after prolonged stimulation, whereas those of cytochrome b-561, carboxypeptidase H and synaptin/synaptophysin (SYN) remain unaltered. Thus, the biosynthesis of chromaffin granules can be regulated in quite sophisticated patterns.

Original languageEnglish (US)
Pages (from-to)321-331
Number of pages11
JournalRegulatory Peptides
Volume32
Issue number3
DOIs
StatePublished - Feb 26 1991

Fingerprint

Chromaffin Granules
Chromogranins
Adrenal Medulla
Neuropeptides
Rats
Neuropeptide Y
Secretogranin II
Chromogranin B
Messenger RNA
Peptides
Oxotremorine
Chromogranin A
Calcitonin Gene-Related Peptide
Nicotine
Carboxypeptidase H
Synaptophysin
Injections
Reserpine
Biosynthesis
Mixed Function Oxygenases

Keywords

  • Adrenal medulla
  • Calcitonin gene-related peptide
  • Chromogranin
  • Cytochrome b-561
  • Neuropeptide Y

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Stimulation of rat adrenal medulla can induce differential changes in the peptide and mRNA levels of chromogranins, neuropeptides and other constituents of chromaffin granules. / Höfle, G.; Weiler, R.; Fischer-Colbrie, R.; Humpel, C.; Laslop, A.; Wohlfarter, T.; Hogue-Angeletti, R.; Saria, A.; Fleming, P. J.; Winkler, H.

In: Regulatory Peptides, Vol. 32, No. 3, 26.02.1991, p. 321-331.

Research output: Contribution to journalArticle

Höfle, G, Weiler, R, Fischer-Colbrie, R, Humpel, C, Laslop, A, Wohlfarter, T, Hogue-Angeletti, R, Saria, A, Fleming, PJ & Winkler, H 1991, 'Stimulation of rat adrenal medulla can induce differential changes in the peptide and mRNA levels of chromogranins, neuropeptides and other constituents of chromaffin granules', Regulatory Peptides, vol. 32, no. 3, pp. 321-331. https://doi.org/10.1016/0167-0115(91)90025-C
Höfle, G. ; Weiler, R. ; Fischer-Colbrie, R. ; Humpel, C. ; Laslop, A. ; Wohlfarter, T. ; Hogue-Angeletti, R. ; Saria, A. ; Fleming, P. J. ; Winkler, H. / Stimulation of rat adrenal medulla can induce differential changes in the peptide and mRNA levels of chromogranins, neuropeptides and other constituents of chromaffin granules. In: Regulatory Peptides. 1991 ; Vol. 32, No. 3. pp. 321-331.
@article{b881ee7c14424134a22e4f8b32d981fa,
title = "Stimulation of rat adrenal medulla can induce differential changes in the peptide and mRNA levels of chromogranins, neuropeptides and other constituents of chromaffin granules",
abstract = "The levels of various components of chromaffin granules were determined in rat adrenals after treatment with several stimulants. After reserpine the levels of calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and chromogranin B but not those of chromogranin A and secretogranin II were elevated. On the other hand, the mRNA of chromogranins A, B and secretogranin II were significantly increased. Treatment with oxotremorine or nicotine (multiple injections for 2 or 3 days) induced analogous changes for peptide and mRNA levels, however, the increases were smaller and for the mRNA less consistent. A single injection of oxotremorine or nicotine raised only the levels of CGRP and NPY and of the NPY mRNA whereas those of the chromogranins and their respective mRNAs remained unaltered. Amongst the membrane proteins only the levels of dopamine β-hydroxylase are increased after prolonged stimulation, whereas those of cytochrome b-561, carboxypeptidase H and synaptin/synaptophysin (SYN) remain unaltered. Thus, the biosynthesis of chromaffin granules can be regulated in quite sophisticated patterns.",
keywords = "Adrenal medulla, Calcitonin gene-related peptide, Chromogranin, Cytochrome b-561, Neuropeptide Y",
author = "G. H{\"o}fle and R. Weiler and R. Fischer-Colbrie and C. Humpel and A. Laslop and T. Wohlfarter and R. Hogue-Angeletti and A. Saria and Fleming, {P. J.} and H. Winkler",
year = "1991",
month = "2",
day = "26",
doi = "10.1016/0167-0115(91)90025-C",
language = "English (US)",
volume = "32",
pages = "321--331",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Stimulation of rat adrenal medulla can induce differential changes in the peptide and mRNA levels of chromogranins, neuropeptides and other constituents of chromaffin granules

AU - Höfle, G.

AU - Weiler, R.

AU - Fischer-Colbrie, R.

AU - Humpel, C.

AU - Laslop, A.

AU - Wohlfarter, T.

AU - Hogue-Angeletti, R.

AU - Saria, A.

AU - Fleming, P. J.

AU - Winkler, H.

PY - 1991/2/26

Y1 - 1991/2/26

N2 - The levels of various components of chromaffin granules were determined in rat adrenals after treatment with several stimulants. After reserpine the levels of calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and chromogranin B but not those of chromogranin A and secretogranin II were elevated. On the other hand, the mRNA of chromogranins A, B and secretogranin II were significantly increased. Treatment with oxotremorine or nicotine (multiple injections for 2 or 3 days) induced analogous changes for peptide and mRNA levels, however, the increases were smaller and for the mRNA less consistent. A single injection of oxotremorine or nicotine raised only the levels of CGRP and NPY and of the NPY mRNA whereas those of the chromogranins and their respective mRNAs remained unaltered. Amongst the membrane proteins only the levels of dopamine β-hydroxylase are increased after prolonged stimulation, whereas those of cytochrome b-561, carboxypeptidase H and synaptin/synaptophysin (SYN) remain unaltered. Thus, the biosynthesis of chromaffin granules can be regulated in quite sophisticated patterns.

AB - The levels of various components of chromaffin granules were determined in rat adrenals after treatment with several stimulants. After reserpine the levels of calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and chromogranin B but not those of chromogranin A and secretogranin II were elevated. On the other hand, the mRNA of chromogranins A, B and secretogranin II were significantly increased. Treatment with oxotremorine or nicotine (multiple injections for 2 or 3 days) induced analogous changes for peptide and mRNA levels, however, the increases were smaller and for the mRNA less consistent. A single injection of oxotremorine or nicotine raised only the levels of CGRP and NPY and of the NPY mRNA whereas those of the chromogranins and their respective mRNAs remained unaltered. Amongst the membrane proteins only the levels of dopamine β-hydroxylase are increased after prolonged stimulation, whereas those of cytochrome b-561, carboxypeptidase H and synaptin/synaptophysin (SYN) remain unaltered. Thus, the biosynthesis of chromaffin granules can be regulated in quite sophisticated patterns.

KW - Adrenal medulla

KW - Calcitonin gene-related peptide

KW - Chromogranin

KW - Cytochrome b-561

KW - Neuropeptide Y

UR - http://www.scopus.com/inward/record.url?scp=0026080387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026080387&partnerID=8YFLogxK

U2 - 10.1016/0167-0115(91)90025-C

DO - 10.1016/0167-0115(91)90025-C

M3 - Article

C2 - 1907749

AN - SCOPUS:0026080387

VL - 32

SP - 321

EP - 331

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 3

ER -